Nitric oxide (NO) is a potent cell‐signaling molecule that plays important roles in diverse range biological processes within various tissues. Almost in every cell of our body, NO is generated by certain enzymes called nitric oxide synthases (NOS). NO is synthesized constitutively in nervous systems and endothelial cells, whereas in some other cells NO production is induced. Excessive NO production is linked to carcinogenesis, septic shock, asthma, stroke, etc. On the other hand, insufficient NO is involved with hypertension, impotence, arteriosclerosis, etc. So, there are dual roles for NO molecule at different tissue level. Since three isoforms of NOS responsible for NO production, an isoform‐specific inhibitor(s) needs to be designed that would not interfere with another NOS. In this regard, we have employed BD‐Clontech made Yeast Two‐Hybrid System to identify the putative proteins that would specifically bind iNOS protein for regulation. An N‐terminal iNOS‐bait protein has been utilized to identify a mammalian cDNA library. Employing all sorts of negative and positive controls necessary to interpret Yeast Two‐hybrid data, we could identify a number protein that shows possible protein‐protein interaction with iNOS. The probable role of these proteins in the regulation or modulation of iNOS function will be discussed.
Nitric oxide (NO) is a potent cell‐signaling molecule. NO can be synthesized by three different (isoforms) nitric oxide synthases (NOS). NO can be physiological in some tissues, but in some cases it may be toxic. Designing the isoform‐specific inhibitor(s) is a key issue so that it would not interfere with physiological NO production. Here, we are investigating human inducible nitric oxide synthase(hiNOS)‐interacting protein(s) that will give us a clue to design such inhibitors of hiNOS. We have employed BD‐Clontech made Yeast Two‐Hybrid System to screen a mouse testis cDNA library and identified a number of candidates for such interaction e.g. Dnaj, fem1b etc.. We are also screening a human testis cDNA library to see if our results look the same.
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