This study aims to determine the effect of tamarind water on fasting blood sugar levels in graduate female students at University of Darussalam Gontor. This study used a pre-experimental study with one group pretest and posttest design without control group with a sample size of 31 people. Samples were given a tamarind water (Tamarindus indica) at 10.00 WIB with a composition of 17 mL for samples with normal nutritional status, and 23 mL with samples with overweight nutritional status. Blood sugar levels were taken before the study was conducted, day 7, and day 14. Blood glucose is measured using peripheral blood draws. To test the difference between fasting blood sugar levels before and after administration of tamarind water, the reapeted ANOVA test was used. Based on the results of statistical tests using the Reapeted ANOVA test, it was found that there was no significant relationship between fasting blood sugar levels before the intervention with on the 7th day, or on the 14th day (p 0.05). It was concluded that giving tamarind water had no effect on fasting blood sugar levels in female graduate students at University of Darussalam Gontor. Penelitian ini bertujuan untuk mengetahui pengaruh air asam Jawa terhadap kadar glukosa darah puasa pada mahasiswa putri pascasarjana Universitas Darussalam Gontor. Penelitian ini merupakan penelitian pra eksperimental dengan rancangan one group pretest and posttest design without control group dengan jumlah sampel 31 orang. Sampel diberikan air asam Jawa (Tamarindus indica) pada pukul 10.00 WIB dengan komposisi 17 mL untuk sampel dengan status gizi normal, dan 23 mL dengan sampel dengan status gizi overweight. Kadar glukosa darah diambil pada sebelum penelitian dilaksanakan, hari ke-7, dan hari ke-14. Glukosa darah diukur menggunakan pengambilan darah perifer. Untuk menguji perbedaan antara kadar glukosa darah puasa sebelum dan sesudah pemberian air asam Jawa, digunakan uji Reapeted ANOVA. Berdasarkan hasil uji statistik dengan menggunakan uji Reapeted ANOVA didapatkan tidak terdapat hubungan yang signifikan antara kadar glukosa darah puasa sebelum intervensi dengan hari ke-7, maupun pada hari ke-14 (p0,05). Dengan demikian dapat disimpulkan bahwa pemberian air asam Jawa tidak berpengaruh terhadap kadar glukosa darah puasa mahasiswi putri Pascasarjana Universitas Darussalam Gontor.