Puberty is a complex developmental stage that elicits a cascade of neuroendocrine changes leading to reproductive maturation. In females, the development of reproductive circuits during puberty culminates in the brain responding positively to ovarian oestradiol (E2), triggering the luteinizing hormone (LH) surge that underlies ovulation. There is mounting evidence that alongside E2, brainderived progesterone (neuroP) is needed for full activation of oestrogen positive feedback: (1) astrocyte-derived neuroP is crucial for fully stimulating kisspeptin release in an in vitro model of adult anterior hypothalamic kisspeptin neurons, 1,2 (2) blocking neuroP synthesis arrests oestrous cyclicity and decreases the LH surge, 3,4 and(3) nuclear progesterone receptor (PGR) signalling in anteroventral
AAV VECTORS IIwhich increased the transduction effi ciency approximately ten-fold compared with the wild-type (WT) AAV2 vector. Combining the best performing S662V mutant with T491V further enhanced the transduction effi ciency by approximately 8-fold. Taken together, these data suggest that high-effi ciency transduction of moDCs by capsid-modifi ed AAV vectors is indeed feasible, which supports the potential utility of these vectors for future human DC vaccine studies.
Dr. Sharpe was a leading eye movement researcher who had also been the editor of this journal. We wish to mark the 10th anniversary of his death by providing a sense of what he had achieved through some examples of his research.
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