Background: Prior to the selection of disinfectants for low, intermediate and high (sterilizing) levels, the decimal reduction time, D-value, for the most common and persistent bacteria identified at a health care facility should be determined.

Prior to selecting disinfectant for low, intermediate and high (sterilizing) levels, the decimal reduction time, Dvalue, for the most common or persistent bacteria identified on a medical device or at a health care facility should be determined. The D-value was determined by inoculating 100 mL of disinfecting solution with 1 mL of a bacterial suspension. At regular intervals, 1 mL aliquots of this mixture were transferred to 8 mL of growth media containing a neutralizing agent, and incubated at optimal conditions for the microorganism. B. stearothermophilus and E. coli were the most resistant bacteria for the disinfecting and sterilizing procedures.

Dedico este trabalho a quatro pessoas: DONA MARIA, minha mãe, pessoa única, que me ensinou a ser forte, e acreditar que tudo posso naquele que me fortalece. EDMUNDO, meu esposo, pelo amor, companheirismo e apolo. CAMILA, minha filha, pelo amor, carinho e paciência. THEREZA CHRISTINA, minha orientadora, pessoa que acreditou na minha vontade e potencial.

Gráfico 6. Cânula de perfusão aórtica ARS020CSTA Gráfico 7. Cânula de perfusão aórtica ARL 2011190 A Gráfico 8. Cânula de perfusão aórtica ARL 241190