Introduction: Pseudomonas aeruginosa (P. aeruginosa), which is a non-fermentative Gram negative opportunistic pathogen, is the leading cause of many infections including pneumonia, wound and urinary tract infection. Aim of the work: To determine the ability of P. aeruginosa clinical isolates to produce biofilm. To detect expression of Lec A gene and biofilm development. Materials and methods: Sample Collection and Microbiological Processing: Samples were collected and sent directly to the microbiology laboratory of
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