Introduction and Aim: We examined the effect of pre- and/or post-infection doxycycline on human nasal epithelial cell viability and SARS-CoV-2 (clinical strain IHUMI-3) replication in vitro.
Materials and Methods: Human nasal epithelial cells, an in vivo SARS-CoV-2 target, were derived from healthy donor nasal epithelial stem/progenitor cells via in vitro differentiation. The cells were exposed to doxycycline at 0, 0.1, 0.5, 1, 5, 10, 50, and 100 ?M before and/or after IHUMI-3 inoculation to determine the optimal inhibitory concentration. Viral replication was evaluated using quantitative reverse-transcription PCR, and doxycycline 50% cytotoxic concentration (CC50) and half-maximal effective concentration (EC50) were calculated. The peak serum concentration (Cmax) resulting from typical oral (100 or 200 mg) or intravenous (100 mg) doxycycline doses was estimated, and the Cmax/EC50 ratio was calculated as an index of potential clinical utility.
Results: Doxycycline exhibited low cytotoxicity (CC50 > 100 ?M) in human nasal epithelial cells and inhibited SARS-CoV-2 replication (EC50: 5.2 ± 3.3 ?M) in a dose-dependent manner when administered pre- and/or post-infection. Reasonable oral or intravenous doses will help achieve effective concentrations in vivo.
Conclusion: Early administration of this well-characterized, safe, and accessible drug may limit person-to-person transmission and prevent progression to severe coronavirus disease.
In December 2019, an outbreak of coronavirus disease 2019 (COVID-19) occurred in Wuhan, China. The disease, which is now a global pandemic, is reportedly associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, we report a case of COVID-19 in a 31-year-old pregnant woman who showed symptoms that included fever, a four-day history of dry cough, and myalgia. Real-time reverse transcriptase-polymerase chain reaction analysis of naso- and oropharyngeal samples was positive for the SARS-CoV-2. A cesarean section was performed during the acute phase of COVID-19; the full-term infant was isolated from his mother and underwent formula feeding. He was healthy and negative for the SARS-CoV-2. This report describes the clinical features, diagnosis, and treatment of the mother’s illness and its effects on her live-born infant.
Introduction and Aim: More than 85% of cervical cancer (CC) cases are reported in developing countries where access to screening is limited. With localized forms of CC, it is possible to achieve good treatment results using radical procedures compared to locally widespread processes.
Methods: This is a retrospective study of 68 patients with stage IIB CC hospitalized from 2013–2022. During the initial treatment and after a full clinical examination, all patients were diagnosed with stage IIB CC, a parametrial and vaginal involvement.
Results: Forty-six patients (74%) received two courses of chemotherapy, 14 patients (23%) received three courses, and 2 patients (3%) received four courses. The tumor became resectable after two courses in 46 patients, after 3–4 courses all patients moved on to the next surgical stage. Of the early postoperative complications, 9 patients (14.5%) had bladder atony, which was resolved by the conservative method.
Conclusion: Thus, there is a certain category of patients with CC, where it is possible to make the tumor into a resectable form, and this will increase the percentage of the surgical component of treatment, which in turn could increase the 5-year disease-free survival rates of patients with stage IIB CC.
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