Background: Spinacia oleracea is widely used as food and in the treatment of urinary calculi, as a laxative, in diffi cult breathing, infl ammation of the liver, and jaundice. However, no studies have been done on its antiinfl ammatory activity. Materials and Methods: To evaluate the anti-infl ammatory activity of the methanolic aqueous fraction (MAF and water extract (WE) of Spinacia oleracea leaf in an acute infl ammation model. was administered in rat hind paw, in chronic infl ammation model, cotton pellet-induced granuloma was performed. Results: The water extract of Spinacia oleracea and its methanolic aqueous fraction at 600 mg/ kg dose showed signifi cant (P < 0.001) inhibition of infl ammation in both acute and chronic anti-infl ammatory models. The potency of the extracts was compared with the standard diclofenac sodium (5 mg/kg). The methanolic aqueous fraction and water extract showed a dose-dependent increase and decrease in catalase content. The highest protein content was found in the water extract.