Summary.-114 biopsy specimens from 70 patients with ovarian carcinoma at all stages of disease were submitted for assessment of clonogenic capacity in agar. A highly significant correlation was found between agar clonogenicity and patient survival after biopsy. However, problems related to inherent tumour heterogeneity, quality of sample and tissue disaggregation indicate that this technique may have limited applicability in the routine assessment of patients. Only 41 biopsy specimens (36%) from 31 patients (44.3%o) complied with the prerequisite criteria for agar clonogenic assessment, namely: (a) the confirmed presence of malignant cells in the biopsy, (b) the ability to prepare a single-cell suspension, and (c) adequate viable cell numbers for assay. Furthermore, although the dominant patterns of agar clonogenic growth could be identified and correlated with stage of disease, the heterogeneity in both initial clonogenic capacity and "self-renewal" capacity assessed by the ability of primary clones to propagate in liquid culture and reclone in agar was too inconsistent for the assay to be used as a prognostic index for the individual patient.
The uptake of 14 C-labeled oleic acid and its incorporation into combined lipids by aortic intimas from normal and cholesterol-fed rabbits has been investigated in vitro. More than five times as much oleic acid was taken up by the atherosclerotic intima as by the normal intima. About twice as much oleic acid was incorporated into phospholipid, and twenty times as much into cholesterol ester by the atherosclerotic intima as by the normal. Lecithin was the major phospholipid synthesized from oleic acid in both normal and atherosclerotic intimas.Radioautographs of the atherosclerotic vessels show that the 14 C-labeled oleic acid and its metabolic derivatives, principally phospholipid and cholesterol ester, were localized in sudanophilic cells in the intima in both early and advanced lesions. It is concluded that intimal foam cells are primarily responsible for the lipid synthesis that occurs in the atherosclerotic lesion.ADDITIONAL KEY WORDS fatty acids atheroma foam cells lipid metabolism arterial wall metabolism radioautography phospholipid cholesterol esterFrom the
The uptake of particles of cholesterol, corn oil and lipoprotein by macrophages in vitro has been studied by electron microscopy and radio‐active counting. The cells were incubated with relatively high concentrations of the lipids for 30 min. at 37° C. and 4° C. Lipoproteins were take up at 4° C. almost as rapidly as at 37° C., whereas the corn oil and cholesterol showed much greater uptake at 37° C. Cholesterol and corn oil passed into the cells via large indentations which became large vesicles. In these some of the corn oil, at least, commenced to be degraded. Lipoproteins predominantly entered small vesicles; some of these fused to form larger ones. Since the particles entering the cells and most of those contained in them were identical with the pure preparations, it was concluded that no significant degradation occurs extra‐cellularly or on the plasma membrane before ingestion.
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