No abstract

Stimulated Raman scattering and the use of bioorthogonal tags provide novel imaging platforms to facilitate the drug discovery process.

Formation of a luminescent device by the Huisgen 1,3-dipolar cycloaddition reaction between a Eu(III) complex and dansyl azide is reported. This reaction is catalyzed by a common biological copper(I) complex [GS--Cu(I)], and the resultant copper(I) catalytic sensor shows a 10-fold enhancement of europium luminescence emission.

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling enables intracellular drug concentrations, distribution and therapeutic response to be measured in living cells.

Towards the identification of characteristic minor components from textiles dyed with weld (Reseda luteola L.) and those dyed with Mexican cochineal (Dactylopius coccus Costa)

Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

We are grateful to the Ministério para a Ciência e Tecnologia, Portugal, for the award of a studentship (PRAXIS XXI BD 13451/97) to Ms Ferreira, the EPSRC for the provision of the ES QIT MS (Grant No. GR/L53618) and the National Museums of Scotland Charitable Trust, for financial assistance. We are also grateful to Dr. R.C. Hartley (University of Glasgow) for the gift of a sample of [2-13C]-labelled quercetin-O-glycoside 12 and to Mr Alan T. Taylor (The University of Edinburgh) for technical assistance.

A new UPLC-PDA MS/MS method has been applied to reference and historical yarns dyed with dyer's greenweed (Genista tinctoria L.). The effect of photo-degradation and textile preparation techniques (such as over-dyeing) on the dye fingerprint was investigated and the results correlated with those obtained from historical samples from the Burrell and Bodleian collections, UK.