L-asparaginase is used in chemotherapy for acute lymphoblastic leukemia and other cancers. L-asparaginase derived from bacterial source triggers immune responses. The current study investigates Solanum nigrum as a novel and latent source of L-asparaginase to minimize immunological reactions. The antitumor activity of SN methanol extract was determined using the potato disc assay. InterPro Chimera and InterPro were used to predict the amino acid sequence of L-asparaginase and its anticancer activity. Purification of the enzyme was carried out to homogeneity of 1.51-fold with a recovery of 61.99%. At optimal conditions of 36.5°C, pH 8.6, and 8.5 g/mL substrate, fruit (crude extract) revealed an L-asparaginase titer of 48.23 U/mL. The molecular weight of the enzyme was calculated to be 32 ± 5 kDa using SDS PAGE. The fruit’s total flavonoids and phenolic contents are 0.42 ± .030 g/mL and 94 ± 1.9 mg CAE, respectively. Anti-tumorigenic efficacy was determined to be 66% against Agrobacterium tumefaciens. Additionally, the extract possesses potent antifungal and antibacterial properties. Molecular docking provided the structural motifs and underlying interactions between L-asparaginase, N-acetylglucosamine, murine, and chitin. SN contains high levels of the enzyme L-asparaginase and phytochemicals, making it a potential source of anticancer drugs.
Background:
Chronic liver injury leads to liver inflammation and fibrosis. This leads towards the activation of myofibroblasts in the liver and secretes extracellular matrix proteins that make the fibrous scar.
Objectives:
The purpose of our study was to characterize the polyphenolic content present in Acacia jacquemontii stem and to evaluate its antioxidant and hepatoprotective activity.
Methods:
The phenolic contents present in Acacia jacquemontii polyphenolic extract (AJPPE) were characterized using high-performance liquid chromatography (HPLC). The hepatoprotective and antioxidant activity of AJPPE was determined through biochemical parameters (ALT, AST, and ALP) lipid profile (TC, TG, HDL, and LDL) antioxidant biomarkers (SOD, LPO, GSH, and CAT), anti-fibrotic activity (collagen deposition) and histopathological analysis.
Results:
HPLC analysis of AJPPE showed the presence of polyphenols including chlorogenic acid, P-coumaric acid, caffeic acid, and kaempferol in a remarkable therapeutic range. Results of in-vivo analysis have shown the significant decrease in the level of lipid profile including LDL (low-density lipoprotein), TC (total cholesterol), and triglycerides; liver function markers (AST, ALT, and ALP); collagen deposition and significantly increased the level of anti-oxidative biomarkers (CAT, SOD, LPO, and GSH) by using AJPPE.
Conclusion:
The above-mentioned results have shown that AJPPE possesses significant anti-oxidative and hepatoprotective effects. Furthermore, histopathological results also supported the antioxidant and hepatoprotective potential of AJPPE.
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