ResumoA castanha-do-brasil pertence à família Lecythidaceae e é uma espécie nativa da Floresta Amazônica com elevado potencial econômico. O presente estudo objetivou avaliar a diversidade genética de AbstractGenetic diversity among Bertholletia excelsa Bonpl genotypes using ISSR molecular markers. The Brazil nut belongs to Lecythidaceae family and is a native species from the Amazon rainforest with high economic potential. This study aimed to evaluate the genetic diversity among Bertholletia excelsa Bonpl genotypes and with naturally occurring in the northern State of Mato Grosso, with ISSR molecular marker. Therefore, we characterized 37 genotypes through eight primers, with a total of 52 amplified loci in all genotypes, being 46 polymorphic. The number of fragments amplified by primer ranged from 5 [Di(AG) 8 were the most divergent and can be used as parents for hybrid formation in the improvement programs. It is recommended to in situ conservation of analyzed genotypes.
Plant growth regulators in the germination of Diospyros hispida A. DC. (Ebenaceae) Seed. Diospyros hispida species is a native fruit that has long germination period, hindering seedling production. The aim of this work was to evaluate the germination of Diospyros hispida seeds under the effect of gibberellic acid and biostimulant applications. Four replicates of 25 seeds per treatment were used. Treatments were: T1 - distilled water, T2, T3 and T4 - GA3 solutions at 100, 150 and 200 mg L-1, respectively, T5 - Immersion for 24 hours in distilled water with aeration, T6, T7 and T8 - Immersion for 24 hours in aerated GA3 solutions at 100, 150 and 200 mg L-1, respectively, T9, T10 and T11 - biostimulant application (containing auxin, gibberellin and cytokinin) at 3, 5 and 7 ml kg-1 of seeds. Germination rate, mean germination time, germination speed index, synchronization index, relative germination frequency, normal seedlings, root length, shoot length and seedling dry mass were evaluated. The effect of gibberellic acid and biostimulant containing auxin, gibberellin and cytokinin negatively influenced Diospyros hispida germination under the conditions of this study.
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