Protozoa of the genus Sarcocystis are among the most prevalent parasitic forms found in pork. In order to rapidly assess the risk that this protozoa produce in human population, a molecular method to detect and identify Sarcocystis suihominis needs to be implemented. The study aimed at characterizing the prevalence of Sarcocystis suihominis in pork obtained in the traditional households through PCR - RFLP method. The material was represented by seventy nine samples of diaphragmatic pillars collected between the period October 2014 - December 2014 from two local sanitary veterinary units from Alba and Cluj. The samples were processed first by trichinelloscopic compression method. All the positive fragments for Sarcocystis spp. were examined through PCR-RFLP method. The method used in the present study utilized the AluI restriction enzyme and had successfully differentiate the Sarcocystis suihominis from other Sarcocystis spp. prevalent in pork meat. The inspection of compressed muscle tissue has detected the presence of Sarcocystis suihominis in a high percentage of investigated samples. The prevalence of Sarcocystis suihominis in the examined samples was 26.58%. According to this result we can affirm that the risk of contamination in humans with Sarcocystis suihominis is high through consumption of contaminated pork meat, raised in the traditional system. In the present scientific research, we demonstrated that this method used for molecular identification of Sarcocystis suihominis has high accuracy and can be successfully applied for obtaining a certain diagnostic.
Aflatoxins are toxic metabolites, generally produced by Aspergillus flavus, A. parasiticus and A. Nomius. They can have immunosuppressive, mutagenic, teratogenic and carcinogenic effects, especially on the liver. The purpose of this study was to evaluate the frequency of AFM1 contamination in milk in an established surveillance area of Romania, during 2013-2017 period. The material was represented by 150 raw milk samples obtained from milk collecting units and breeding units in Cluj county. The samples were analyzed by ELISA method. Measurable levels of AFM1 were present in 28% of the milk samples examined. The level of AFM1 in the positive samples ranged between 0,010 and 0,089 μg/kg. Of the total samples, only two were found non-compliant with the EU regulatory limit (0.05 μg/kg). All the positive samples were detected in 2013. In the 2014-2017 period, the level of aflatoxin M1 in milk was undetectable. The contamination risk of AFM1 in milk may increase with feeds being stored in improper conditions of humidity and temperature. This risk can be significantly reduced by using aflatoxin uncontaminated feeds.
Gram negative bacteria have the greatest capacity to spoil the meat if kept under aerobic conditions, therefor the member s of the genera Pseudomonas, Acinetobater, Psychrobacter and Moraxella, will form the dominant microflora. The purpose of this study was to assess the contamination level with psychrotropic microflora of bovine carcasses in order to prove how the results could be used to improve the slaughter process. The research was carried out between January-December 2015, in two slaughterhouses from North West Romania. The research material was represented by 144 meat samples (slaughterhouse A, n=72, slaughterhouse B, n=72). Weekly, 3 samples were collected from refrigerated carcasses, and examined for total psychrotrophic counts (TPC), Enterobacteriaceae, Pseudomonas, Aeromonas and Yersinia. From statistical processing of recorded data was established that mean log TPC from the surface of refrigerated carcasses has presented different values, ranged between 3.70±0.20 log CFU/ cm 2 and 6.90±0.43 log CFU/cm 2 . Initial surface microflora of bovine carcasses was represented by germs from the following genera: Staphylococcus, Micrococcus, Aeromonas, Acinetobacter, Pseudomonas, Yersinia, Serratia, Hafnia, Proteus and Escherichia. Microbiological hazard assessment reveals the key role of psychotropic microorganisms in the spoilage of meat, if the monitoring system of the slaughtering process is not functioning properly.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.