Transparency of algorithmic systems entails exposing system properties to various stakeholders for purposes that include understanding, improving, and/or contesting predictions. The machine learning (ML) community has mostly considered explainability as a proxy for transparency. With this work, we seek to encourage researchers to study uncertainty as a form of transparency and practitioners to communicate uncertainty estimates to stakeholders. First, we discuss methods for assessing uncertainty. Then, we describe the utility of uncertainty for mitigating model unfairness, augmenting decision-making, and building trustworthy systems. We also review methods for displaying uncertainty to stakeholders and discuss how to collect information required for incorporating uncertainty into existing ML pipelines. Our contribution is an interdisciplinary review to inform how to measure, communicate, and use uncertainty as a form of transparency.
Podocytes are specialized cells that contribute critically to the normal structure and function of the glomerular filtration barrier. Their depletion plays an important role in the pathogenesis of glomerulosclerosis. Here, we report generation of a genetic model of conditional podocyte ablation and regeneration in zebrafish using a bacterial nitroreductase strategy to convert a prodrug, Metronidazole, into a cytotoxic metabolite. A transgenic zebrafish line was generated that expresses a green fluorescence protein (GFP) and the nitroreductase fusion protein under the control of the podocin promoter Tg(podocin:nitroreductase-GFP). Treatment of these transgenic zebrafish with Metronidazole results in podocyte apoptosis, a loss of nephrin and podocin expression, foot process effacement, and a leaky glomerular filtration barrier. Following Metronidazole washout, proliferating cells were detected in the glomeruli of recovering transgenic fish with a restoration of nitroreductase-GFP fluorescence, nephrin and podocin expression, a reestablishment of normal foot process architecture and glomerular barrier function. Thus, our studies show that zebrafish podocytes are capable of regenerating following depletion and establish the Tg(podocin:NTR-GFP) fish as a new model to study podocyte injury and repair.
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