Introduction: World Health Organization (WHO) data indicate that SARS-CoV-2 infection has already caused more than 6.8 million deaths since the beginning of the COVID19 pandemic. Currently, there are only three antiviral molecules (remdesivir, tocilizumab, baricitinib) approved by the American FDA for clinical use against COVID19. Studies show that carrageenans are considered potential inhibitors of several enveloped viruses, such as SARS-CoV-2, requiring further in vitro analyzes to understand their mechanism of action.Objectives: Therefore, the present study intends to identify carrageenans with effect on viral replication and compare the effects of these polysaccharides of commercial origin to those produced directly by the algae Kappaphycus alvarezzi.Methodology: This red macroalgae is being cultivated in the context of a biorefinery, in marine farms in the Santa Catarina-Brazil, where there is a high fluctuation in temperature and salinity, specific to this region. These conditions tend to generate modifications in the production profile of carrageenans isoforms (kappa, lambda and iota). For this comparison, cytotoxicity assays are conducted to assess cell viability at different concentrations of the analyzed molecules, as well as in vitro antiviral assays in Calu-3 (human lung epithelial cell lines), virucide assays and ultrastructural evaluation of cell monolayers after infection and treatments.Results: Preliminary results showed that the commercial carrageenans lambda and iota inhibited SARS-CoV-2 replication in 80%, when evaluated 24 hours of treatment at 10 μM without cytotoxic effects. New studies are being carried out to obtain the selectivity index (CC50/EC50) of these molecules in Calu-3. In addition, we are evaluating the carrageenans modulation of cytokines associated with the SARS-CoV-2 pathogenesis.
Conclusion:Thus, the success of this project brings perspective to the development of new formulations, such as nasal and/or oral sprays, based on the bioprospecting of the K. alvarezzi biomass, which until then has been discarded after the production of the biofertilizer.
No contexto da sociedade líquido-moderna onde palavras como velocidade, flexibilidade, imediatismo, desapego, mobilidade e rentabilidade circulam comprimindo o tempo numa “cultura da urgência” (BAUMAN, 2007; SENNETT, 2012), julga-se pertinente indagar: que vestígios de cooperação e de solidez relativos à experimentação do tempo poderiam se fazer notar? A fim de responder essa indagação, buscou-se levantar e analisar a existência de vestígios de cooperação e de solidez relativos à experimentação do tempo em um CTG (Centro de Tradições Gaúchas). Elaborou-se uma pesquisa qualitativa exploratória, realizada por meio de um estudo de caso singular, em que a unidade de análise é o 35 CTG, onde foram realizadas entrevistas narrativas com os associados. O estudo permitiu conhecer quais são os elementos, alguns dos rituais e a forma como estes operam no contexto do caso do 35 CTG, tornando palpável o contexto onde encontramos os vestígios da cooperação e solidez.
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