Prom the Westem Poi nsylz.tr,i ia Hospital, Institute of Pathology, Pittsburgh.With K. F. Semsroth, formerly of this Institute, one of us (Mellon) initiated experiments that have been substantially amplified and extended through the assistance of the above collaborators. The few original experiments were the outgrowth of unpublished studies by one of us (Mellon), attempting to correlate the antiseptic action of iodine with its wound healing power-the inference appearing j ustified that it might be partially accounted for by its neutralization of certain toxic products of protein decomposition, originating perhaps from the tissues or from microorganisms. In support of this point of view Eppinger,' found that iodine neutralized the toxicity of putrifying meat pulp. He inferred from his experiments that the toxicity was due to certain unsaturated protein cleavage products, of which allylamine is a type. From our standpoint, the pertinent pathological effect of allylamine as shown by him is a marked increase of endothelial permeability.It was, therefore, natural to see whether allylamine applied to wounds would delay their healing, and to observe whether the effect on the endothelium was reflected in detectable morphologic alterations in the vessel walls. Both effects were observed, the latter being of a noteworthy character, consisting in their close similarity to the experimental arteriolitis of Klinge2 as shown in his hyperergic vascular lesions, and to the necrotizing arteriolitis and malignant nephrosclerosis of Fahr.3Twenty-one rabbits were eniplc.yed. The allylamine was applied to small areas denuded of skin, as well as given intradermically. Control administration of NH,OH was employed. Each type of sslution, the allylamine and the NH,OH, was used in both buffered and unbuffered form. All buffered solutions had a p H of 7. The doses of allylamine ranged from 0.2 cc. to 0.5 cc. (intradermically) of solutions varying in concentration from 1% up to one to one million ( 1 P ) .
Special media. The three synthetic media employed are shown in table 1. All experiments were conducted with 5 ml of single strength medium per culture tube. 1 We are indebted to Lederle Laboratories, Incorporated, for the synthetic folic acid, and to Merck and Company for the pyridoxal hydrochloride and the pyridoxamine dihydrochloride.
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