PHOSPHOLIPIDS IN OX LIVER 51 rancid phosphatides, whose amount is small in relation to the total phospholipid. 3. Degraded phosphatides were present in the fraction expected to contain phosphatidic acid. It was therefore considered impossible either to exclude or confirm the presence of naturally occurring phosphatidic acid. 4. The bearing of these facts on the isolation of phosphatidic acid reported by Hubscher & Clark (1960) is discussed. 5. Linoleic acid (70 %) and linolenic acid (12-15 %) were the main fatty acids in ox-liver cardiolipin. 6. Phosphatidylinositol isolated in yields of 64-70 % of the total inositide contained stearic acid as virtually the only saturated acid (45-55 %) and polyenoic acids in varying proportion as the main unsaturated components. 7. Successive fractions of liver lecithin with decreasing iodine values obtained by chromatography on silicic acid contained similar proportions of saturated acids and decreasing proportions of polyenoic acids. I wish to thank the British Empire Cancer Campaign for support of this work.
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