Blastocystis sp. is the most common unicellular, luminal parasite in humans and animals. It has been associated with diarrhea and irritable bowel syndrome. This article describes the bibliometric research of articles published in Web of Science (WoS) database on Blastocystis sp. over a 51-year period. The VOSviewer visualization methodology was used for this study. Institutions, nations, international collaborations, journals, articles, authors, keywords, co-authors, co-citations, and citation rates from the WoS database formed the data of this study. A total of 1066 documents were found in the WoS database. Of which 773 were articles and only those publications were analyzed. The articles were cited 22,034 times in total and the mean Hirsch (H) index was 74. The countries with the highest number of articles were as follows: USA (n = 86; 11.125%), Singapore (n = 71; 9.185%), and Malaysia (n = 70; 9.056). Since 2000, there have been a limited number of articles and since 2014 there has been a minimum of 34 publications per year. The highest number of articles was published in 2021 (n = 76) and the highest number of citations (n = 3408) was in 2021. The leading affiliation according to the number of published articles was the National University of Singapore (9.185%), University Malaya (7.245%), and Nara Women’s University (5.304%). The USA, Singapore, and Malaysia have led scientific production on Blastocystis sp. Efforts should be made to help them develop interdisciplinary scientific research networks with these countries on Blastocystis sp.
Objective: Anaerobic bacteria, which make up an important part of normal body flora, may lead to serious life-threatening infections. Since isolation and identification of anaerobic bacteria require time-consuming, sensitive, and difficult methods, they can only be performed in certain clinical laboratories. For this reason, diagnosis and treatment of anaerobic infections are delayed and drug resistance is observed due to empirical treatment. New methods that will enable the early identification of these bacteria will help reduce the duration of treatment and mortality rates due to anaerobic infections. Method: In this study, it is aimed to design magnetic nanoparticles attached to N-methyl-D-glucamine (Mag-NMDG) to catch anaerobic bacteria for rapid identification. Mag-NMDG nanoparticles were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). Results: Mag-NMDG nanoparticles were applied to gram positive and gram negative anaerobic bacteria such as Actinomyces odontolyticus, Prevotella buccae, Veillonella parvula, Bifidobacterium dentium and Bacteroides fragilis isolated from culture media. The binding of bacteria to Mag-NMDG was determined by microscope images, McFarland values, and MALDI-TOF MS identification scores. Conclusion: As a result of this study, it was concluded that the Mag-NMDG nanoparticles could be used to isolate anaerobic bacteria directly from samples. Thus, it is foreseen that many time-consuming and troublesome steps in the isolation and identification stages can be eliminated.
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