The antibacterial and antifungal activities of the bulb and flower of Allium staticiforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAE/100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities. Video Clip of Methodology: Disc Diffusion Method: 3 min 04 sec:  Full Screen   Alternate

Pyracantha coccinea Roem. (scarlet firethorn) is known as a medicinal plant that can be up to 3 m in height with lots of thorns, grown generally in South and Southeast Europe and Turkey. Pyracantha coccinea Roem is being used in folk medicine since its fruits have diuretic, cardiac and tonic properties. To determine the antioxidant level of the different parts of the plants takes interest in the current researches. In this study it has been aimed to evaluate the antioxidant and antimicrobial activity levels, Fe 3+ reduction power and the total phenolic amount of the ethanolic extracts of the fruits of Pryacantha coccinea using DPPH scavenging, the disc diffusion, reduction power and Folin-Ciocalteu methods, respectively. IC 50 value, which represents the 50% scavenging value of DPPH radical of prepared fruit extracts using the Maceration technique, is evaluated to be 36.53 µg/mL The total phenolic content is determined to be 199.6 mgGA/100g and Fe +3 ions are reduced to a certain amount for various concentration levels (from 20 to 100 µg/mL). From the current research we have also found that the fruits of Pyracantha coccinea have no any antimicrobial activity. As a general result of the current study it has been concluded that the fruits of Pyracantha coccinea have extremely higher level of antioxidant activity depending upon the phenolic contents, showing that they can be used in various food and health applications.

In this study, antioxidant and antibacterial activities of Brassica nigra, Linum usitatissimum, Salvia hispanica, Nigella sativa, Chenopodium quinoa seeds prepared with ethanol were determined. Antioxidant capacity has been measure by evaluating DPPH radical scavenging activity level and antibacterial activities were investigated by using the disc diffusion method. Highest antibacterial activity level has been detected for the seed of N. sativa. As a result of this study there exist no any antibacterial effect in the seeds of B. nigra and L. usitatissimum. The extract of N. sativa seed has shown an inhibition zone of 14.5 mm on Staphylococcus aureus, whereas this value has been detected to be 10.5 mm for both on the seeds of Enterococcus faecalis and Staphylococcus epidermidis. IC 50 value showing the 50% scavenging value of DPPH radical has been determined to be 51 µg/mL for the seed extrafct of B. nigra as the highest one between the all seed samples searched. All seeds used in the current study which are known to be functional food have shown certain level of antioxidant activity.

In this work, antimicrobial effects of the root, stem, leaf and flower parts extracts with ethanol and acetone of Ferulago galbanifera species against Escherichia coli, Staphylococcus epidermidis, Enterococcus faecalis Candida albicans, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium strains were investigated by using the disc diffusion method. In addition to that, antioxidant activity of F. galbanifera ethanolic extracts was measured using the DPPH method, as well as their total phenolic content using the Folin-Ciocalteu's phenol reagent technique. To our results, ethanolic extracts of leaf from F. galbanifera were found to have antimicrobial effect against the all microorganisms, whereas the acetonic extracts of leaf has shown antimicrobial effects to some microorganisms other than Staphylococcus epidermidis, E. faecalis, C. albicans species. Extracts obtained from the root and the flower parts of the plant had no antimicrobial effect on the test microorganisms. The antioxidant activity level was found to be in the following order (from the highest to the lowest): flower, leaf, stem and rood; 16.32, 215, 244, 323(μg/mL), respectively. The highest total phenolic content obtained from the parts of the plant F. galbanifere was for the root, while the lowest was for its leaf part.

The increase in the occurrence of the multidrug-resistant bacteria is the cause of need in the discovering new antimicrobial substances and antimicrobial combination drugs. The aim of this study was to investigate the synergistic interaction between polar fractions of pomegranate (Punica granatum L.) peel and Amoxicillin trihydrate against Enterococcus faecalis. In the first step of the study, polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) were prepared. Next, disc diffusion method was used for determination of the synergic effect.Here, the results indicated that the mixture of prepared polar fractions of pomegranate peel and Amoxicillin trihydrate showed synergistic interaction against E. faecalis. This study determined that polar fractions of pomegranate peel (ethyl acetate, butanol, distilled water) improved the effectiveness of Amoxicillin trihydrate against E. faecalis. Pomegranate peel may have potential applications in the new antibacterial therapy combinations due to its easy accessibility, therapeutic properties and being low cost. Results of the present study may be beneficial for further studies about the characterization of phytochemical compounds that are responsible for the improvement of the antibiotic effectiveness.