15 isolates (39.4%) of Proteus mirabilis were obtained from a total of 38 patients with urinary tract infections in Al-Diwaniyah Teaching Hospital. All isolates were diagnosed with phenotypic, microscopic and biochemical tests. PCR technique was also used as a confirmatory test to investigate the 16S rRNA gene (product size 239bp) in Proteus mirabilis , where the results showed the presence of the gene in all isolates .On the other hand ,the detection of biofilm formation in Proteus mirabilis isolated from patients urinary tract infection was done using the tubes method .The results showed that 10 (75%) isolates were capable of producing the biofilm, where the value of the optical density of the formed biofilm ranged from 0.064-0.411 nm.Then, the effect of diefferent temperatures (25,30 37,40) ℃ on the Biofilm Formation in Proteus mirabilis was studied, Where the most efficient bacteria were selected in the production of the biofilm and the test was carried out .The results showed that the bacteria were unable to form the biofilm at 25℃ either at 3040℃, the biofilm is very thin at 37℃ and 40℃, it becomes a thick biofilm.The results of the current study showed that the temperature has a significant effect on the production of the biofilm and that the thickness of the biofilm increases with increasing temperature.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.