A novel kinetic method for measuring catalase activity in biological samples was evaluated. The principle of the current method is based on the oxidation effect of unreacted hydrogen peroxide (H 2 O 2 ) on pyrogallol red (PGR) using the catalytic effects of molybdenum. The decrease in the absorbance of PGR in the presence of H 2 O 2 with time from 0.5 to 4.5 min was directly proportional to the concentration of H 2 O 2 , and, in turn, directly proportional to catalase activity. Erythrocyte lysate homogenates were used to measure catalase activity and the results of the current method were significantly correlated to those of the ammonium peroxovanadate method. The 3.1% within run and 4.7% between run coefficients of variation indicated the high precision of the present novel method. The validation process confirmed that the diagnostic method is appropriate for different types of biological samples. Here, we describe a rapid, relatively easy, and reliable method for measuring catalase activity. The assay could be applied as a diagnostic tool and is suitable in research contexts. A novel kinetic method for measuring catalase activity in biological samples was evaluated. The validation process confirmed that the diagnostic method is appropriate for different types of biological samples. The assay could be applied as a diagnostic tool and is suitable in research contexts.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.