Fluorescence quenching is a powerful technique used to
obtain information
about the dynamic changes of proteins in complex macromolecular systems.
In this work, graphene is shown to be a very efficient quencher of
fluorescence molecules where the quenching effect was one order of
magnitude higher than that of gold. The fluorescence intensity was
distance-dependent where increasing the distance between the fluorescence
molecule and the graphene surface from 4 to 7 nm increased the fluorescence
intensity by a factor of 7.5. This type of distance dependence suggests
a nonradiative nature in the energy transfer between the graphene
and the fluorophore due to the excitation of an exciton.
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