As agriculture industrializes, concentrated animal feeding operations (CAFOs) are becoming more common. Feces from CAFOs is often used as fertilizer on fields. However, little is known about the effects manure has on the soil microbiome, which is an important aspect of soil health and fertility. In addition, due to the subtherapeutic levels of antibiotics necessary to keep the animals healthy, CAFO manure has elevated levels of antibiotic resistant bacteria. Using 16s rRNA high-throughput sequencing and qPCR, this study sought to determine the impact of swine CAFO manure application on both the soil microbiome and abundance of select antibiotic resistance genes (ARGs) and mobile element genes ( erm(B) , erm(C) , sul1 , str(B) , intI1 , IncW repA ) in agricultural soil over the fall and spring seasons. We found the manure community to be distinct from the soil community, with a majority of bacteria belonging to Bacteroidetes and Firmicutes. The soil samples had more diverse communities dominated by Acidobacteria, Actinobacteria, Proteobacteria, Verrucomicrobia, and unclassified bacteria. We observed significant differences in the soil microbiome between all time points, except between the spring samples. However, by tracking manure associated taxa, we found the addition of the manure microbiome to be a minor driver of the shift. Of the measured genes, manure application only significantly increased the abundance of erm(B) and erm(C) which remained elevated in the spring. These results suggest bacteria in the manure do not survive well in soil and that ARG dynamics in soil following manure application vary by resistance gene.

In semi-arid protected areas, artificial waterholes ensure that water is locally available to animals for extended periods. However, artificial waterholes may limit animal movement, which contributes towards habitat deterioration. Challenges of artificial water provisioning worsen in the presence of ecosystem engineers like African elephants , capable of transforming environments. Camera traps were used to monitor elephant visitation at 21 artificial waterholes in the Kruger National Park, South Africa. We also assessed if water quality parameters influenced elephant preference for certain waterholes. There were no significant correlations between elephant abundance and water physicochemical properties. However, there was a strong negative correlation between elephant abundance and levels of in water. Our findings suggest that elephants avoid drinking water with high levels of faecal microbial loads. Whereas most studies addressing animal management in protected areas consider waterholes as homogeneous units, we posit that water quality could also determine local landscape use and movement patterns of key species like elephants, a finding with relevant implications in reserve management practices.

Immunocastration relies on the vaccine-mediated stimulation of an immune response to gonadotropin-releasing hormone (GnRH) in order to interrupt spermatogenesis. This approach offers a less painful alternative to traditional castration approaches but the current, commercially available options require multiple doses of vaccine to maintain sterility. Thus, a series of pilot studies were conducted to determine the feasibility of a single-dose immunocastration vaccine implant. These five studies utilized a total of 44 Holstein bulls to determine the optimal vaccine composition and validate the ability of a stainless-steel subcutaneous implant to deliver a vaccine. Outcome measures included the duration of implant retention, scrotal dimensions and temperature, implant site temperature, anti-GnRH antibodies, and serum testosterone concentration. Over the course of several studies, anti-GnRH antibodies were successfully stimulated by vaccine implants. No significant treatment effects on scrotal dimensions or testosterone were detected over time, but changes in spermatogenesis were detected across treatment groups. Results indicate that a single-dose implantable immunocastration vaccine elicits a humoral immune response and could impact spermatogenesis in bulls. These findings provide opportunities for the refinement of this technology to improve implant retention over longer periods of time. Taken together, this approach will offer producers and veterinarians an alternative to physical castration methods, to improve animal welfare during routine livestock management procedures.

Eucheuma striatum and Eucheuma spinosum, are red algae of commercial value because of their carrageenin content, to an increasing extent are cultivated in the Philippines. The influence of environmental factors on growth rate and photosynthesis of these seaweeds were studied through observations in their natural biotope in seaweed farms, from in-situ experiments, and photosynthesis experiments in an incubator. While difference in light intensity and temperature play only a minor role, the algae were found out to be sensitive to low salinity and to the amount of dissolved gases and nutrients in the surrounding seawater. The algae can only be cultured in areas where there is sufficient current but to a cer-tain extent lack of current can be compensated by exposing the plants to wave action. This leads to certain conclusions regarding the most suitable method of commercial Eucheuma culture. Eucheuma can be successfully cultured in small rafts or in floating baskets in areas where there is insufficient current for the use of fixed nets or strings. There is evidence that-the CO2 -02 metabolism in the surrounding water plays an important role in Eucheuma growth. Eucheuma harvest can be further increased by introducing more nutrients. Fertilizing with phosphate caused a bigger increase in growth rate than with nitrogen. The method of spraying plants after temporarily removing them from the water, found to be more effective than fertilizing solutions by the use of clay pots. The presence of a bacterial disease, locally referred to as "ice-ice", was recognized as a clear indication that certain environment conditions were unfavourable for Eucheuma culture. Low salinity, in the first place, but also lack of current favors the attack of "ice-ice". Finally, it was found that Eucheuma adapts itself to its culture site in such a way that after maintaining seedlings in an area for a sufficient length of time, the cultured plants showed better growth rates and were resistent to unfavorable conditions than plants recently transferred to the area.

Caulerpa racemosa, a benthic siphonaceous algae has its natural habitat in the sublittoral zone of tropical areas. In the Philippines, since a few years, it is cultivated on commercial scale in flooded ponds in the intertidal mangrove zone. Unlike in their natural habitats, Caulerpa in these ponds is subjected to sometimes considerable changes in salinity (rain, evaporation, tem-perature and light (Insolation), and in pH). The response of Caulerpa to these factors and the comparison of the ecological conditions, both in the natural habitats and the cultured ponds have been investigated. The experiments showed that the observed changes of the studied parameters normally range within the physiological limits of Caulerpa. Under extreme conditions, however, detri-mental effects can occur. Ways of controlling such factors are discussed.