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Fibrinogenolysis and prolonged blood coagulation have been observed following large intravenous doses of crystalline trypsin ( 1 ) .2 The present study was undertaken to evaluate further the effects of intravenous crystalline trypsin upon the protein components of the coagulation mechanism and the fibrin content of artificially induced intravascular thrombi, in 60 rabbits and 25 dogs. A striking observation in this study was the lack of untoward reactions, including shock, when the rate and concentration of injected trypsin were kept within carefully calculated limits. MATERIALS 1) Sixty rabbits whose average weight was 2 kg. were fed rabbit food pellets and given water ad libitum.2 Quick (2). Fibrinogen was determined by the method of Greenberg (3). The Innerfield, Angrist, and Benjamin method (4) of antithrombin titer determination was used. Factor V was measured by the method of Stefanini (5); and fibrinolysis assay was made by the Conley, Ratnoff, and Hartmann method (6). Determinations of coagulation time were made by the Lee-White method (7). Blood volume was estimated following administration of labeled albumin (8). Continuous measurements of clotting viscosities were made with a RichRoth Ultra-viscosimeter (9). PROCEDURE 1) Rabbits. Blood samples were obtained from non-anesthetized rabbits by cardiac puncture, before and at varying intervals during infusion of trypsin. Sections of an ear vein thrombus were obtained for histologic study prior to, during, and after completion of each intravenous infusion.2) Dogs. Trypsin infusions were given to dogs who had been anesthetized with 6 per cent Nembutal. Blood samples were obtained prior to, and during the course of the infusion. In securing 1049

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