Rotational diffusion of eosin-5-maleimide-labeled band 3 was measured in erythrocyte membranes at pH 9.4-10.4. Band 3 was found to be more mobile in this pH range than at pH 7.5. Similar results were obtained with spectrin-actin-depleted membranes, where it was further shown that ankyrin is the only detectable protein released from the membrane at pH 10. Further experiments were performed at pH 7.5 to investigate the effects of rebinding purified ankyrin and/or band 4.1 to ghosts stripped of skeletal proteins. Ankyrin was found to reduce band 3 rotational mobility, but band 4.1 had no effect. A fluorescence binding assay revealed that fluorescein isothiocyanate-labeled ankyrin had similar binding parameters to those reported previously using 125I labeling. Finally, the rotational mobility of purified band 3 reconstituted into lipid bilayers was determined before and after ankyrin binding. The results of these reconstitution experiments were globally analyzed, assuming the existence of two populations of band 3 with different correlation times. The faster correlation time is consistent with that expected for either dimers or compact tetramers of band 3. Ankyrin binding reduces the proportion of band 3 contributing to the faster component. This result demonstrates that ankyrin promotes the association of band 3 into more slowly rotating complexes independently of any other components of the erythrocyte membrane. It has been reported that ankyrin contains two binding sites for band 3 [Michaely, P., & Bennett, V. (1995) J. Biol. Chem. 270, 22050-22057]. The results of the present study are thus explained by the ability of ankyrin to cross-link band 3 into larger diameter complexes. Cross-linking by ankyrin in part accounts for the slow components in the anisotropy decays of band 3 in the erythrocyte membrane. Other factors which probably influence band 3 aggregation include the membrane "fluidity" and protein concentration.

The effect of antibodies to glycophorin A on the rotational diffusion of band 3 in human erythrocyte membranes was investigated by transient dichrosim. Three antibodies that recognize different epitopes on the exofacial domain of glycophorin A all strongly reduce the rotational mobility of band 3. The effect is at most only weakly dependent on the distance of the epitope from the membrane surface. The degree of immobilization obtained with two of the antibodies, BRIC14 and R18, is very similar to that produced by antibodies to band 3 itself. Similar results were obtained with membranes stripped of skeletal proteins. Fab fragments and an antibody to glycophorin C had no effect on band 3 rotational mobility. These results rule out a mechanism whereby band 3 rotational immobilization results from enhanced interactions with the membrane skeleton that are mediated by a conformational change in glycophorin A. Rather, they strongly indicate that the antibodies to glycophorin A cross-link existing band 3-glycophorin A complexes that have lifetimes that are long compared with the millisecond time scale of the transient dichroism measurements.

Microaggregation of band 3 proteins in hereditary ovalocytic membranes was investigated by rotational diffusion measurements and by electron microscopy. It was previously shown that band 3 in ovalocytic membranes has decreased rotational mobility compared with band 3 in normal cells (Tilley, L., Nash, G.B., Jones, G.L. and Sawyer, W.L. (1991) J. Membr. Biol. 121, 59–66). This result could arise from either altered interactions with cytoskeletal proteins or from band 3 microaggregation. In the present study it was found that removal of spectrin and actin from the membrane had no effect on the rotational mobility of ovalocytic band 3. Additional removal of ankyrin and band 4.1, as well as cleavage of the cytoplasmic domain of band 3 with trypsin, did enhance band 3 mobility, as is the case in the membranes from normal cells. However, the rotational mobility of ovalocytic band 3 was always considerably less than that of normal band 3 under the same conditions. Scanning electron microscopy and low power electron micrographs of freeze-fracture replicas revealed that the surfaces of ovalocytes were more irregular than those of normal erythrocytes. At higher magnification, numerous linearly arranged intramembranous particles were observed on the P-faces of freeze-fractured ovalocytes but not on normal cells. These clusters consist of straight or slightly curved lines of 10–15 particles in single rows. From these results it is deduced that the reduced rotational mobility of band 3 in ovalocytes is a consequence of the formation of microaggregates, which are very probably induced by the mutation in the membrane-bound domain of ovalocytic band 3.