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In this study the removal of Mn (II) ions from wastewater using magnetite nanomaterial was investigated. Some factors influencing the wastewater treatment process were studied such as: treatment time, pH and the concentration of Mn (II) ions from wastewater. The results showed that using magnetite nanomaterial adsorbent lead to a wastewater treatment efficiency higher than 97%. Langmuir and Freundlich models were applied to describe the adsorption process. The correlation coefficients (R2) showed that both models are applicable to the experimental data obtained.

Various types of functional yogurts were obtained from normalized milk (with normalized lipid content) and a standardized probiotic consortium of probiotic bacteria named ABY3. All the types of yogurts obtained contained prebiotics from black or red rice; malt of barley, rye, wheat; or wheat bran. The physico-chemical analyses of all the functionalized products obtained showed that all of them met the quality standard for yogurt products. However, the sensorial analyses showed that the products obtained from black and red rice were of very good quality. The biological analyses indicated that all the types of products contained live probiotic bacteria, but wheat bran and red rice could increase their numbers. Tests performed on tumor cell line Caco-2 with corresponding postbiotics revealed cytotoxicity greater than 30% after 48 h of exposure in the case of yogurts obtained from milk with 0.8% lipid content and red rice or blond malt of barley. In the case of yogurts derived from milk with 2.5% lipid content, only the variants that contained blond malt of rye or wheat became cytotoxic against the Caco-2 cell line.

A spent biomass, which results from the biopharma industry, is stabilized and functionalized by biosorption with microelements. The efficiency of this new biomaterial was tested in two experiments: (1) In a mixture with soil to determine its effects of the germination capacity of cereals and vegetables, and (2) in a formulation of mixed fertilizers to determine its influence on the development and production of the two types of vegetables. The results obtained during germination experiments performed in pots showed that at a biomass concentration less than 20%, the germination output was greater than 95% and the germination index was almost 1. The experiments performed in land on vegetables (including Solanum lycopersicum and Capsicum annuum) featured six types of fertilizers formulated with new biomaterials. The obtained results indicated that two types of fertilizers (N 10:0:0 and NP 5:5:0), which were formulated with functionalized biomass and featured the microelements Co, Cu, Fe, Mn, and Zn, exhibited significant effects when compared with vegetables cultivated on unfertilized soil surfaces (the untreated variant). The studies regarding the effect of the new fertilizers obtained based on spent biomass from biopharma industry indicate the following: (a) This material, even if it is stabilized and functionalized, cannot be used as such as a germination substrate for vegetables; in addition, it cannot be introduced into soil together with cereals seeds (during the autumn work), because the germination can be affected negatively; (b) the functionalized biomass can be used in the formulation of different types of fertilizers; if these fertilizers are introduced into soil with the autumn plowing, then they may have a positive influence on the yield of some species of vegetable, such as Solanum lycopersicum and Capsicum annum. The new fertilizers have a major environmental impact due to: (1) Removal of waste, which results from pharmaceutical biosyntheses, with significant impact on soil pollution, due to its storage in the form of waste dumps, on the soil; (2) recovery and reinsertion into the natural circuit of nutrients like C, N,

Brewing yeast biomasses may be converted into valuable products, including supplements for ruminant diets. This paper presents experimental data on a nutraceutical suspension formulation containing post-fermentation brewing yeasts and the brewing yeast strain, Saccharomyces pastorianus ssp. carlsbergensis W34/70, which was intended to be administered to ruminants. The brewing yeast biomass is a component of oral suspensions designed as dietary supplements that prevent digestive acidosis in ruminants. The brewing yeast strain W34/70 was cultivated using molasses (M) and glucose (G) as carbon sources and was lyophilized at different freezing temperatures (0�C, �10�C, �20�C, �30�C, and �40�C). The post-fermentation brewing yeast biomass was spray dried (AAY) and lyophilized (ALY). Both dried yeast biomasses were analyzed in terms of wettability using a contact angle (CA�) evaluation at the solid/liquid interface. Suspensions were subsequently formulated and tested. Their physical and aggregative stability was established in connection with controlled sedimentation and flocculation. The assays were performed following quality by design (QbD) principles. The critical process parameters (CPPs) corresponding to the technological process of yeast production, as well as the critical quality attributes (CQAs) for suspension formulations, were pointed out as preamble determinants when designing oral nutraceuticals destined to be included in ruminant diets.

Yeast (including brewing yeast) and yeast-based preparations derived from bioprocesses or agroindustrial byproducts represent valuable feed additives and ingredients for ruminants. The optimization of brewing yeast biotechnological processing through fermentation mediated by the brewing yeast strain Saccharomyces pastorianus ssp. carlsbergensis W34/70 was investigated. The cultivation conditions (temperature, pH, carbon source, and nitrogen source) were selected and designed according to a Taguchi fractional experimental plan, with four factors on three levels, and their influence on the evolution of the bioprocess of obtaining the brewing yeast biomass was evaluated. The dependent variables were the yeast biomass amount in wet form, yeast biomass amount in dried form after lyophilization, dried yeast biomass wettability assayed through the contact angle (CA), protein content (PC), and dry matter content (DS). The effects that the experimental conditions had on the system responses were visualized in tridimensional space using the response surface methodology, and the combination of biotechnological parameters that ensured process quality and robustness was then determined using the Taguchi technique through its performance indicator, i.e., the signal-to-noise ratio. By optimizing the biotechnological parameters, this study provides a valuable contribution in the area of brewing yeast biomass processing, with the aim of producing probiotic yeast for ruminant nutrition.