Hypericum empetrifolium Willd. subsp. empetrifolium is an evergreen small shrub with small elongated decorative leaves and small yellow flowers in inflorescences, characterized for several pharmaceutical properties. In the present study, a first approach on the sexual and asexual propagation of this species was performed. Seeds, subjected to different types of pre-treatments [soaked in tap water; 50, 100 or 150 mg L-1 GA3 for 30 min and no treatment (control)], cultured for germination in petri dishes at 5, 10, 15, 20, 25, and 30 °C. Seed germination was only affected by temperature and the best result was obtained at 15 °C (71.2%). A significant interaction was found between pre-treatments and incubation temperature with the highest germination percentage (82%) occurred when the seeds soaked in 100 mg L-1 GA3 solution for 30 min and incubated at 20 °C constant temperature. Moreover, the germination speed was fastest from 20 to 25 °C (T50= 9.84 and 9.56 days for 20 and 25 °C, respectively). For asexual propagation, apical stem cuttings were taken at four different periods (4 seasons) and treated with IBA at concentrations of 0, 1000, 2000 and 3000 mg L-1. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Winter was the most appropriate season for cuttings collection (100% rooting percentage) and dipping in 1000 or 2000 mg L-1 IBA (72% and 73%, respectively) was the best rooting hormone treatment.
The aim of this research was to examine the effect of Cd on leaves dry matter (LDM), root dry matter (RDM) and on Cd concentration in leaves (Cd-leaves) and roots (Cd-roots) of purple coneflower (E. purpurea) grown in an acid and in a neutral substrate. A completely randomized block design with four treatments (0-control, 1, 2, and 5 mg Cd L-1) and six replications for each treatment and each substrate (acid, neutral) was conducted in pot experiments. Cd concentration in leaves grown in acid substrate as well as in roots of plants grown in neutral substrate increased at Cd rates greater than 2 mg Cd L-1. Cd concentration in roots of the plants grown in neutral substrate raised with increasing doses of Cd. In general Cd concentration in the roots and leaves of purple coneflower grown either in acid or in neutral substrate was affected by Cd applications.
The propagation with seeds and stem cuttings of Teucrium brevifolium were investigated in order to facilitate the use of the species in floricultural practice and as a medicinal plant. The seeds after they subjected to different types of treatments (dipping in H2SO4 for 15 min or in H2SO4 for 15 min and GA3 1000 mg l-1 for 10 min, 24 or 48h and untreated) cultured for germination in vitro at 5, 10, 15, 20, 25, and 30 °C. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Seed germination was very low (2%) at temperatures of 15-25 °C, while chemical treatment with the concentrated H2SO4 for 15 min, increased germination to 8-10% at the same temperatures. Asexual propagation of the species resulted in a 52.5-61.5% rooting percentage, in all seasons except winter. Treatment with IBA 2000 mg l-1 increaseds rooting percentage.
The effect of cadmium (Cd) on growth and Cd accumulation in shoots and roots St John’s wort (Hypericum empetrifolium) was studied over three months in a greenhouse. Plants were cultivated in pots containing a uniform mixture of either acid or alkaline substrate consisting of peat and perlite (1:1 v/v). The pots were arranged in a completely randomized block design within two groups (acid substrate and alkaline substrate) with four Cd treatments (0-control, 1, 2, and 5 mg Cd L-1) and six replicates per treatment. Cadmium was applied as CdSO4*8/3H2O. The total amount of Cd applied per pot was 260 ml, corresponding to 0.26, 0.52, and 1.3 mg Cd per pot for doses 1, 2, and 5 mg L-1, respectively. No visual symptoms of toxicity or nutrient deficiency, as well as no differences in plant height were observed in response to Cd application, irrespective of the growth stage or substrate. There were also no differences in height development rate between the plants grown in an acidic or alkaline substrate. Cd accumulation in shoots and roots increased with increasing concentrations of applied Cd and was higher in the acidic substrate. Thus, St John’s wort plant is a Cd accumulator, especially in an acidic environment, and this in combination with its high tolerance to Cd, makes it a suitable species to remove Cd from cadmium-contaminated sites. However, for its use in the preparation of medical products, St John’s wort must be grown in a Cd-free soil so as not to pose a risk to human health. Cd extraction by (DTPA-TEA) can be employed to predict Cd accumulation in this plant.
Nickel is an essential trace element for plants, but excessive Ni levels in the soil can result in toxicity to plants. The aim of this research was to examine the effect of Ni on shoots dry matter (SDM), roots dry matter (RDM) and on Ni concentrations in SDM (Ni-SDM) of mojito and lavender plants. A completely randomized block design with five treatments (0, 5, 10, 20 and 40 mg Ni L-1) and five replications for each treatment and plant species was conducted in pot experiments. Nickel was applied to the pot medium as NiCl26H2O. No visible toxic or inhibitory symptoms were observed on the plants due to the increasing rates of Ni applications. The SDM and RDM were not affected by Ni in the studied plants. Ni-SDM of mojito and lavender raised with increasing rates of Ni above 20 and 10 mg Ni L-1, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.