Development ofrninimal invasive diagnostics methods is ofgreat importance in today healthcare. The present work deals with the measurements ofglucose concentration in small drops, its volume being in the range of 1 tL and its glucose concentration being in the range of 1 -1 0 mM. The aim of the work was to develop robust quantitative method of glucose measurement in small volumes of interstitial fluid obtained after laser shot or by other low invasive procedures. The commercially available AmlexTM Red glucose assay kit was used in the present work. The glucose is detected by enzyme-coupling reactions resulting to formation of fluorescence dye-resorufin. The diagnostic paper was used as a reaction medium. The fluorescence of dye in diagnostic paper was measured with the use of fiber optics spectrometer. It was shown that dye fluorescence correlates fairly well with glucose concentration. The method may be modified to involve cholesterol concentration measurement as well.
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