A method for the determination of substrate flux through the pentose cycle was developed employing [l‐14C]glucose in experiments with perfused rat livers. The method consists first of a kinetic analysis which differentiates between the production of 14CO2 from [l‐14C]glucose via the pentose cycle and via the citrate cycle and, second of a calculation of the specific radioactivity of the hexose monophosphate pool from measured rates of glycolysis and the specific radioactivity of lactate released into the perfusate. The method was validated by experiments comparing the results of tracer infusions with [l‐14C]glucose, [6‐14C]glucose and [3‐14C]pyruvate. In livers from fed rats perfused with 10 mM glucose, the rate of substrate flux through the pentose cycle was around 0.2 μmol ×min−1× g−1; it was about 20% of the substrate flux via glycolysis. The kinetic data were inconsistent with the existence of an L‐type pentose cycle in liver.
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