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Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

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Up to now, the caryophyllid cestode Atractolytocestus huronensis Anthony, 1958, a parasite of common carp, has attracted little attention in Germany. Based on recent publications from the Czech Republic and Hungary, it appears probable that this cestode may be increasingly common in Germany. There is a strong connection between the occurrence of A. huronensis and imports of common carp from the Czech Republic and southern Germany. Although in most cases no clinical alterations in parasitized carp have been observed, care should be taken to avoid further dissemination and to prevent possible losses in commercial pond farming. KEY WORDS: Caryophyllaeidae · Atractolytocestus · Morphology · Prevalence · Common carp · GermanyResale or republication not permitted without written consent of the publisher

Influences of different culture media and growth surfaces on morpho-functional characteristics of equine endometrial epithelial cells (EEC) in vitro were investigated in order to optimize culture conditions. Therefore, the results were compared to the endometrium in situ. Equine EEC were cultured using different media with varying serum components and additives: foetal bovine serum plus additives (MFBS+A), foetal bovine serum (MFBS), horse serum 1 (MHS1), horse serum 2 (MHS2) and horse serum 3 (MHS3). Uncovered and Matrigel™-covered cell culture inserts, as well as cell culture flasks were used. Cultured cells were examined cytomorphologically (haemalaun-eosin staining, giemsa staining), cytochemically (alcian blue staining) and immunocytochemically (oestrogen receptor (ER) α, progesterone receptor, proliferating cell nuclear antigen, inhibin-α, cytokeratin 8, 18, 19, vimentin, α-actin, desmin, transforming growth factor (TGF)-α,-β1,-β2,-β3). Further, tissue samples of the uteri from which the cells were isolated were examined histopathologically (haemalaun-eosin staining), histochemically (alcian blue staining) and immunohistochemically, using the above-mentioned antibodies. Regardless of the used media and the state of the endometrial cycle at point of isolation, two morphologically different cell types and three growth patterns were found. None of these facts led to apparent differences concerning immunolabelling. Whereas growth surfaces, duration of culture and state of endometrial cycle at point of isolation did not cause any obvious influences on the immunolabelling of EEC in vitro concerning the utilized antibodies, the culture media did. Especially cells grown in MFBS+A exhibited characteristics comparable to uterine glandular epithelia in situ concerning cytokeratin 8, 18, 19, TGF-β1,-β3 and ERα.

Der Aufsatz untersucht den Roman Schimmernder Dunst über CobyCounty des deutschen Gegenwartsautoren Leif Randt auf die in diesem Text verhandelten Körperbilder. In Schimmernder Dunst über CobyCounty wird die Geschichte eines Ortes und dessen Protagonisten Wim, eines 26-jährigen Literaturagenten, der sein ganzes Leben in CobyCounty verbracht hat und – geplagt von Ängsten vor dem Älterwerden und somatischen Beschwerden – mit dem Beginn einer neuen Lebensphase hadert, erzählt. Der Aufsatz versucht zu zeigen, dass vor allem eine Zuhilfenahme der Simulations- und Simulakrumstheorie Baudrillards bei der Deutung dieses schwierigen Romans helfen und eine Baudrillard-Lektüre und die entsprechende Verarbeitung für diesen Roman und die Gestaltung der epistemischen Regeln der erzählten Welt bei genauer Lektüre geltend gemacht werden kann. In der Gestaltung der fiktiven Welt von CobyCounty sind sechs Elemente (seelische Nostalgie, Authentizitätsbegehren, Untergangsfantasien, Auferstehung des Figurativen, Ökonomie des Realen, Abklatsch und Kopie), die dieser Diagnose Baudrillards entlehnt sind, auszumachen, die Randt um die Kontrastfolie einer spezifischen Körperlichkeit ergänzt.

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