Much of an animal’s health status, life history, and behavior are dictated by
interactions with its endogenous and exogenous bacterial communities. Unfortunately,
interactions between hosts and members of their resident bacterial community are often
ignored in animal behavior and behavioral ecology. Here, we aim to identify the nature of
host–microbe interactions in a nonmodel organism, the African social spider
Stegodyphus dumicola. We collected and identified bacteria from the
cuticles of spiders in situ and then exposed spiders to bacterial
monocultures cultures via topical application or injection. We also topically inoculated
spiders with a concomitant “cocktail” of bacteria and measured the behavior of spiders
daily for 24 days after inoculation. Lastly, we collected and identified bacteria from the
cuticles of prey items in the capture webs of spiders, and then fed spiders domestic
crickets which had been injected with these bacteria. We also injected 1 species of
prey-borne bacteria into the hemolymph of spiders. Only Bacillus
thuringiensis caused increased mortality when injected into the hemolymph of
spiders, whereas no bacterial monocultures caused increased mortality when applied
topically, relative to control solutions. However, a bacterial cocktail of cuticular
bacteria caused weight loss and mortality when applied topically, yet did not detectibly
alter spider behavior. Consuming prey injected with prey-borne bacteria was associated
with an elongated lifespan in spiders. Thus, indirect evidence from multiple experiments
suggests that the effects of these bacteria on spider survivorship appear contingent on
their mode of colonization and whether they are applied in monoculture or within a mixed
cocktail. We urge that follow-up studies should test these host–microbe interactions
across different social contexts to determine the role that microbes play in colony
performance.
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