The effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l -1 6-benzyladenine (BA) with 0.5 mg l -1 a-naphthalene acetic acid (NAA) and 0.2 mg l -1 BA with 0.1 mg l -1 NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l -1 BA with 0.5 mg l -1 NAA and 4 mg l -1 BA with 0.1 mg l -1 NAA, respectively, resulted in the highest frequencies of shoot organogenesis.

Recent interest in the use of wood substrates in horticulture crop production has justified the need for determining fertilizer requirements in these substrates compared with traditional pine bark (PB) and peatmoss substrates. The objective was to determine the response of japanese holly (Ilex crenata Thunb. ‘Compacta’) and azalea (Rhododendron obtusum Planck. ‘Delaware Valley’) grown in a pine tree substrate (PTS) (trade name WoodGro™) or milled PB to fertilizer rate. Pine tree substrate is produced from freshly harvested loblolly pine trees (Pinus taeda L.) that are delimbed, chipped, and ground in a hammer mill to a desired particle size. Japanese holly plants were grown in 2.8-L containers in the fall of 2005 and again in the spring of 2007 with the addition of azalea. Plants grown in PTS or PB were fertilized by incorporating Osmocote Plus fertilizer (15N–3.9P–10K) at rates of 3.5, 5.9, 8.3 or 10.6 kg·m−3 for japanese holly and 1.2, 3.5, 5.9, or 8.3 kg·m−3 for azalea. After 3 months, shoot dry weights were determined for japanese holly and azalea. Japanese holly root dry weights were determined for both experiments, and substrate CO2 efflux (μmol CO2 m−2·s−1) was measured on the treatments at the end of the experiment using a LI-6400 soil CO2 flux chamber. In 2005, japanese holly shoot dry weights of PTS-grown plants were comparable to plants grown in PB at the 8.3 kg·m−3 fertility rate, and shoot dry weights of PTS-grown plants were higher than PB at the 10.6 kg·m−3 rate. In 2007, japanese holly and azalea shoot dry weights of PTS-grown plants were comparable to PB plants at the 5.9 kg·m−3 fertilizer rate. Both japanese holly and azalea achieved shoot growth in PTS comparable to shoot growth in PB with ≈2.4 kg·m−3 additional fertilizer for PTS. Substrate CO2 efflux rates were higher in PTS compared with PB indicating higher microbial activity, thereby increasing the potential for nutrient immobilization in PTS.

No abstract

Studies were conducted to evaluate the effect of water application medium moisture deficit, water application rate, and intermittent application on water application efficiency {[(amount applied - amount leached)/amount applied] x 100} of spray stake-irrigated, container-grown plants. Pine bark-filled containers were irrigated to replace moisture deficits of 600, 1200, or 1800 ml; deficits were returned in single, continuous applications of 148, 220, or 270 ml·min-l. Efficiency was unaffected by application rate but decreased with increased medium moisture deficit. In the second experiment, container medium at a 600-ml deficit was irrigated with 400 or 600 ml (6570 and 100% water replacement, respectively); deficits were returned in a single, continuous application or in intermittent 100-ml applications with 30-min intervals between irrigations. Application efficiency was greater with intermittent irrigation (95% and 84% for 400- and 600-ml replacement, respectively) than with continuous irrigation (84% and 67% for 400- and 600-ml replacement, respectively). In the third experiment, pine bark was irrigated with 600 ml water (100% replacement) in 50-, 100-, or 150-ml aliquots with 20, 40, or 60 min between applications in a factorial design. Efficiency increased with decreasing application volume and increasing time between applications. Highest efficiency (86%) was achieved with an irrigation regimen of 50-ml applications with at least 40 min between applications, compared to 62% for the control treatment (a single, continuous application of 600 ml). Our results suggest that growers using spray stakes would waste less water by applying water intermittently rather than continuously.

Regulatory and economic incentives to improve water and fertilizer use efficiency have prompted the nursery industry to seek new and advanced techniques for managing the production of ornamental crops. The development of best management practices, especially with regard to fertilizer and irrigation management, is largely based on research that looks at season-long trends in water and nutrient use. Understanding how water moves through a substrate during a single irrigation event may allow for the refinement of recommended best management practices that improve water and fertilizer use efficiency in container-grown plant production systems. Therefore, a study was conducted to characterize the movement of irrigation water at three growth stages [4, 9, and 17 weeks after transplanting (WAT)] throughout the production cycle of Ilex crenata Thunb. ‘Bennett’s Compactum’ that were container-grown in a bark-based substrate alongside fallow (i.e., without a plant) containers. Tensiometers were placed at three horizontal insertion depths and three vertical heights throughout the substrate profile to detect changes in matric potential (ψ; kPa), during individual irrigations. At 4 WAT, the pre-irrigation ψ in the upper substrate profile was 12.3 times more negative (i.e., drier) than the substrate near the container’s base and 6.0 times more negative than the middle of the container. This gradient was decreased at 9 and 17 WAT as roots grew into the lower portion of the substrate profile. On average, water began to drain from the base of containers 59.9 s ± 1.0 se and 35.7 s ± 1.3 se after irrigation commencement for fallow containers and plant-containing treatments, respectively, indicating channeling through the substrate of plant-containing treatments. A pattern of plant water uptake by roots induced a gradient in the substrate’s pre-irrigation moisture distribution, where portions of the substrate profile were relatively dry where plant roots had taken up water. Consequently, the application of water or fertilizer (i.e., fertigation) through irrigation has the potential to be highly inefficient if applied under dry substrate conditions where channeling may occur. Therefore, water application using cyclic irrigation or substrate moisture content (MC) thresholds (not letting MC fall below an undetermined threshold where channeling may occur) may improve water application efficiency. Furthermore, fertigation should occur when the substrate MC in the upper portion of the container is higher than the pre-irrigation MCs observed in this study to minimize the occurrence of channeling. The effect of root growth should also be taken into account when seeking the proper balance between pre-irrigation substrate MC and irrigation application rate to reduce the risk of unwanted channeling.