High molecular weight DNA has become a necessary resource with the advancement of long-read sequencing from Nanopore ONT and PacBio SMRT. Often times excessive treatment with phenol-chloroform and prolonged periods of centrifugation results in sheared DNA below 10 kilobases. DNA extracted from viruses is notably less stable than that of cellular organisms, and is often heavily sheared upon extraction. Likewise, kits designed specifically for viral DNA extraction tend to return very low yields of DNA. This protocol seeks to subvert the shearing of typical DNA extractions by combining the process with pulsed-field gel electrophoresis while also maintaining high yields.
Tabanus nigrovittatus
greenhead populations contain two supergroups of
Wolbachia
endosymbionts, members of supergroups A and B. Analysis of the greenhead microbiome using next-generation sequencing revealed that the majority of bacterial species detected belonged to
Gammaproteobacteria
, with most of the samples also showing the presence of
Spiroplasma
, a member of the
Mollicutes
phylum also known to infect insects.
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