This SOP validates an inductively coupled plasma mass spectrometry (ICP-MS) procedure for the determination of biometals and trace elements in hydrothermal fluids and sediments. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and trace elements that can be used by a set of microbial proteins containing redox-sensitive transition metals as their catalytic core. Due to the high variability of these samples, we have developed this protocol taking into account the special features of the matrices analyzed. Our methods focus primarily on a subset of trace metals, Trace metals such as Fe, Co, Ni, Mo, W, V and Cu referred to as biometals, used by biology as cofactors in proteins. An ICP-MS 7900 Agilent system was used, with calibration curves linear in the 0.01 to 100 μg/L concentration range.
This SOP describes an ion chromatography (IC) procedure for the major cations and anions in hydrothermal fluids. Hydrothermal fluids are aqueous solutions with a wide range of temperature, salinity, pH and ion species that can be used by microbial metabolism as electron donors and electron acceptors. Due to the high variability of the environmental physical-chemical parameters in these samples, we have developed this protocol taking into account the special features of the matrices analyzed. An Eco IC Metrohm system equipped with a conductivity detector was used. Calibration curves are linear in the 0.1 to 10 mg/L concentration range of cations Ca2+, Na+, K+, Mg2+, NH4+ and anions Cl-, Br-, NO3-, NO2-, SO42- , HPO42- .
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