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Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells. We propose a Wide-Field Multi-Parameter FLIM method (WFMP-FLIM) aimed to monitor continuously living cells under minimum light intensity at a given illumination energy dose. A powerful data analysis technique applied to the WFMP-FLIM data sets allows to optimize the estimation accuracy of physical parameters at very low fluorescence signal levels approaching the lower bound theoretical limit. We demonstrate the efficiency of WFMP-FLIM by presenting two independent and relevant long-term experiments in cell biology: 1) FRET analysis of simultaneously recorded donor and acceptor fluorescence in living HeLa cells and 2) tracking of mitochondrial transport combined with fluorescence lifetime analysis in neuronal processes.

Occlusions are commonplace in man-made and natural environments; they often result in photometric features where a line terminates at an occluding boundary, resembling a "T". We show that the 2-D motion of such Tjunctions in multiple views carries non-trivial information on the 3-D structure of the scene and its motion relative to the camera. We show how the constraint among multiple views of T-junctions can be used to reliably detect them and differentiate them from ordinary point features. Finally, we propose an integrated algorithm to recursively and causally estimate structure and motion in the presence of T-junctions along with other point-features.

We present a variational approach to the problem of registering planar shapes despite missing parts. Registration is achieved through the evolution of a partial differential equation that simultaneously estimates the shape of the missing region, the underlying "complete shape" and the collection of group elements (Euclidean or affine) corresponding to the registration. Our technique applies both to shapes, for instance represented as characteristic functions (binary images), and to grayscale images, where all intensity levels evolve simultaneously in a partial differential equation. It can therefore be used to perform "region inpainting" and to register collections of images despite occlusions. The novelty of the approach lies on the fact that, rather than estimating the missing region in each image independently, we pose the problem as a joint registration with respect to an underlying "complete shape" from which the complete version of the original data is obtained via a group action.