This study evaluated the antifungal susceptibility profile and the production of
potential virulence attributes in a clinical strain of Candida
nivariensis for the first time in Brazil, as identified by sequencing the
internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of
the rDNA. For comparative purposes, tests were also performed with reference strains.
All strains presented low planktonic minimal inhibitory concentrations (PMICs) to
amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed
elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to
fluconazole resistance. Adherence to inert surfaces was conducted onto glass and
polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing
cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal
strains were able to bind both tested surfaces and form biofilm, with a binding
preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%)
in biofilm production by our C. nivariensis strain using both
methodologies. This reduction was also observed for CAS and POS, but only in the XTT
assay. All strains were excellent protease producers and moderate phytase producers,
but lipases were not detected. This study reinforces the pathogenic potential of
C. nivariensis and its possible resistance profile to the azolic
drugs generally used for candidiasis management.
Background:The Trichosporonaceae family comprises a large number of basidiomycetes widely distributed in nature. Some of its members, especially Trichosporon asahii, have the ability to cause human infections. This ability is related to a series of virulence factors, which include lytic enzymes production, biofilm formation, resistance to oxidising agents, melanin and glucuronoxylomannan in the cell wall, metabolic plasticity and phenotypic switching. The last two are poorly addressed within human pathogenic Trichosporonaceae.Objective: These factors were herein studied to contribute with the knowledge of these emerging pathogens and to uncover mechanisms that would explain the higher frequency of T. asahii in human infections.
Methods:We included 79 clinical isolates phenotypically identified as Trichosporon spp. and performed their molecular identification. Lactate and N-acetyl glucosamine were the carbon sources of metabolic plasticity studies. Morphologically altered colonies after subcultures and incubation at 37°C indicated phenotypic switching.
Results and Conclusion:The predominant species was T. asahii (n = 65), followed byTrichosporon ovoides (n = 1) and Cutaneotrichosporon arboriforme (n = 1). T. asahii isolates had statistically higher growth on lactate and N-acetylglucosamine and on glucose during the first 72 h of culture. T. asahii, T. inkin and T. japonicum isolates were able to perform phenotypic switching. These results expand the virulence knowledge of Trichosporonaceae members and point for a role for metabolic plasticity and phenotypic switching on the trichosporonosis pathogenesis.
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