Sloe (Prunus spinosa L.) extracts are a good source of natural bioactive compounds, including phytosterols. Phytosterols are known to be applied in the treatment of various prostate diseases. The in vitro antiproliferative activity of sloe ethanolic extracts (flower, leaf, and fruit), collected from three areas in Bosnia and Herzegovina, were investigated against human prostate cancer cell lines PC-3 and DU145 using MTT assay. β-sitosterol, campesterol and stigmasterol were quantified by HPLC-PDA analysis using Symmetry C18 chromatographic column. The results of the analysis proved the presence of phytosterols, mostly β-sitosterol in all extracts. All extracts possess antiproliferative activity. The highest activity against PC-3 and DU145 was gathered from leaf extracts obtained by different extraction methods (microwave-assisted extraction and ultrasound-assisted extraction). To the best of our knowledge, no other studies have presented results on antiproliferative activity of ethanol sloe extracts. Based on these results, further investigation should be recommended on other cancer cell lines as well.
Magnesium is an essential element and the intracellular divalent cation involved in many biochemical functions. People with magnesium deficiency must increase their intake of magnesium, usually in the form of various supplements. A common form of magnesium supplement widely available in pharmacies is magnesium oxide (MgO). In this work, the content of MgO was determined in pharmaceutical supplementations using spectrophotometry, based on the reaction between magnesium ions and eriochrome black T at a wavelength of 535 nm. The analysed content of MgO ranged from 360.5 to 386.5 mg MgO, which corresponds to the daily Mg recommended values (300 to 400 mg).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.