The problem of infertility affects approximately 20% of married couples, and the number of people affected by this problem is still increasing[5,6]. Developed countries are characterized by the prevalence of primary infertility, while in developing countries there is a high rate of secondary infertility[8,9]. The most common cause of marital infertility is obstruction of the fallopian tubes, which occurs in 30-35% of women and requires surgical intervention. However, both sexes are dominated by immunological and hormonal disorders (25%-30% of cases)[3,4]. The basic examination in which male fertility is determined is the semen analysis, while in women, many tests should be performed in order to make a diagnosis: anamnesis, gynecological examination, ultrasound performed repeatedly during the menstrual cycle (ovulation assessment), hormonal and immunological tests, post-coital tests, bacteriological cultures, hysterosalpingography or laparoscopy [20]. In vitro fertilization is the most effective of all methods of infertility treatment. In vitro fertilization can be performed using the classical method by adding prepared sperm to the egg cells or by microinjection of the sperm into the egg cell[21].
The role of diet and supplementation in the prevention and treatment of autism spectrum disorders There is evidence indicating the positive effects of dietary intervention in the prevention and treatment of autism spectrum disorders. There are studies linking maternal vitamin D deficiency with ASD in offspring. Children and adolescents on the autism spectrum are also supposed to have significantly lower concentrations of vitamin D than healthy children and adolescents. This indicates the role of vitamin D supplementation in the prevention and treatment of ASD(5). Diets most commonly used in the therapy of autism spectrum disorders are: dairy-free and gluten-free, specific carbohydrate diet, low phenol, low oxalate and ketogenic diet(4). The most popular of them are dairy-free and gluten-free. However, studies conducted by Dr. James Adams attribute the highest effectiveness to a diet of specific carbohydrates(7). After applying the ketogenic diet, improvements in concentration, learning ability and social behavior were observed(1,26). However, it should be remembered that the scientific literature contains a small number of reliably conducted studies confirming the effectiveness of individual diets and supplementation in the prevention and treatment of ASD. Therefore, the positive impact of dietary intervention is still referred to as "potential". In addition, the use of certain diets may be associated with side effects, such as vomiting and hypoglycemia in the case of a ketogenic diet (1,28) and underweight in the case of elimination diets(16). Therefore, the introduction of dietary changes for therapeutic purposes should be carried out under the constant supervision of doctors and dieticians.
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