Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in Mexico. Biotechnological studies of its use are lacking. The objective of this work was to establish a cell suspension culture (CSC) of E. platycarpa, determine the phytochemical constituents by spectrophotometric and gas chromatography‒mass spectrometry (GC‒MS) methods, evaluate its antifungal activity, and compare them with the intact plant. Friable callus and CSC were established with 2 mg/L 1-naphthaleneacetic acid plus 0.1 mg/L kinetin. The highest total phenolics of CSC was 15.6 mg gallic acid equivalents (GAE)/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 µg quercetin equivalents (QE)/g dry weight. The GC‒MS analysis showed that the dichloromethane extracts of CSC, sapwood, and heartwood have a high amount of hexadecanoic acid (22.3‒35.3%) and steroids (13.5‒14.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (~23.4%) and β-sitosterol (~43%), and leaf extracts presented β-amyrin (16.3%). Methanolic leaf extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Compared with the intact plant, dichloromethane and fatty hexane extracts of CSC exhibited percentages of inhibition higher for Sclerotium cepivorum: 71.5% and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggests that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.
We present the synthesis of ZnO nanostructures grown by the hydrothermal method using the rapid microwave heating process. First, 10 mM solutions of zinc acetate dihydrate and 1-propanol were three cycles spin coated on glass substrates at 2000 rpm by 70 s, 90 s and 90 s, respectively. Second, nanostructures were then grown by dipping the substrates in a solution of zinc nitrate hexahydrate, polyethyleneimine (PEI), hexamethylenetetramine and ammonia. The hydrothermal process were carried out with a commercial microwave at 300, and 600 W power settings during 20 min. The structural and morphological properties were investigated using X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). The influence of the ammonium concentration in the morphology of the nanostructures was analyzed.Keywords: ZnO, nanostructures, XRD, SEM, spin coating, ammonium, hydrothermal process.
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