This study was performed to determine the effect of the addition of L. essential oil (EO) (at 0.01% v/v) and of the packaging material (brown and transparent glass or PET) on the oxidative stability of Algerian extra-virgin olive oil (EVOO) stored for 90 days at 25 ± 2 °C under continuous exposure to fluorescent light (~ 900 lux). Control and enriched EVOO was analysed after 30, 60 and 90 days for various parameters. Bio-enrichment of EVOO with EO combined with packaging in brown glass enabled maintenance of the highest amounts of chlorophyll and carotenoids after 90 days of accelerated photo-oxidation. The lowest total phenols content was found in EVOO without EO packed in transparent glass. EO enrichment and brown packaging preserved higher levels of antioxidant activity but could not preserve the oxidation indices until the end of the period of light exposition.
Background: In response to consumer demand for novel and healthy foods, the presence in the market of olive oils (OOs) flavored with different plants, spices, herbs or fruits is increasingly common. All these flavoring agents have been used over the years due to its content in compounds with biological activities. Aim: The aim of this study was to investigate the potential role of the addition of S. montana EO at 100 ppm (0.01%, v/v), known for its high content of bioactive compounds, good flavor, and aroma in improving oxidative stability and quality profile of EVOO subjected to conditions causing accelerated oxidation (Light storage at 900 lux). Materials and methods: The S. montana EO chemical components were identified using Gas Chromatography–Mass Spectrometry (GC/MS). Enriched and non-enriched EVOO samples were examined as function of time (30, 60 and 9 days) of display for different quality indices. Results: Using GC/MS analysis of S. montana EO: thymol (28.36%), carvacrol (17.45%), p-cymene (10.91%), trans-caryophyllene (5.54%), ɤ-terpinene (5.03%) and geraniol (4.50%) were identified. The results highlighted that the enrichment with S. montana EO led to lower values of lipid oxidation indicators (K232, K270, peroxide value) and higher concentration of antioxidants (total phenols and pigments). In sum, the use of bioenrichment methods could be a sustainable solution for the promotion of the quality characteristics of EVOO in Algeria.
Keywords: Bioenrichment, Satureja montana L., Essential oil, Extra virgin olive oil, Display, Quality stability.
The aim of the study was to determine the phenolic and flavonoid content of essential oils (EOs), chloroform and ethanolic extracts of 12 Algerian Thymus species and evaluate their antioxidant and antifungal activities. EOs (1.73 ± 0.30–15.00 ± 1.24 μg/mg), chloroform extracts (33.8 ± 2.42–160.93 ± 3.88 μg/mg) and ethanol extracts (27.01 ± 3.56 –148.46 ± 4.40 μg/mg) showed considerable phenolic content. Flavonoids values of chloroform extracts ranged between 3.39± 0.17 and 20.27 ± 0.29 μg/ml while ethanolic extracts values ranged between 2.81 ± 0.11 and 26.64 ± 0.18 μg/mg. Results of DPPH showed that EOs, chloroform and ethanolic extracts exhibited strong radical scavenging activity (IC50 = 21.75 ± 6.54–338.22 ± 2.99 μg/ml, 22.91 ± 5.59–90.93 ± 1.36 μg/ml, and 33.51 ± 5.72–103.80 ± 4.54 μg/ml, respectively). Inhibition of β-carotene bleaching was potentially performed by all EOs (66.48 ± 2.41–94.06 ± 2.68 %), chloroform extracts (68.98± 1.58–95.30± 1.99%), and ethanolic extracts (62.15 ± 2.51–92.36± 1.15%). The antifungal activity of EOs and extracts was tested using the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). The EOs (0.1 ± 0.00 mg/ mL – 1.06 ± 0.46 mg/mL), chloroform (0.1 ± 0.00 mg/ mL –1.06 ± 0.46 mg/mL) and ethanol (0.1 ± 0.00 mg /mL–1.6 ± 0.00 mg/mL) showed remarkable antifungal activity against mycotoxigenic Aspergillus genera. The MFC of EOs (1.0 ± 0.34 mg/mL and > 4.8 mg/mL) , chloroform (0.26 ± 0.11 mg/mL and > 1.6 mg/mL) and ethanol (0.2 ± 0.00 mg/mL and > 1.6 mg/mL) were fungicidal in nature higher than MICs. The findings of the study indicated that Thymus spp. EOs and extracts could be used as natural alternatives for food industry.
Background: In response to consumer demand for novel and healthy foods, the presence in the market of olive oils (OOs) flavored with different plants, spices, herbs or fruits is increasingly common. All these flavoring agents have been used over the years due to their content in compounds with biological activities. Aim: The aim of this study was to investigate the potential role of the addition of S. montana EO at 100 ppm (0.01%, v/v), known for its high content of bioactive compounds, good flavor, and aroma in improving oxidative stability and quality profile of EVOO subjected to conditions causing accelerated oxidation (Light storage at 900 lux). Materials and methods: The S. montana EO chemical components were identified using Gas Chromatography–Mass Spectrometry (GC/MS). Enriched and non-enriched EVOO samples were examined as function of time (30, 60 and 90 days) of display for different quality indices. Results: Using GC/MS analysis of S. montana EO: thymol (28.36%), carvacrol (17.45%), p-cymene (10.91%), trans-caryophyllene (5.54%), ɤ-terpinene (5.03%) and geraniol (4.50%) were identified. The results highlighted that the enrichment with S. montana EO led to lower values of lipid oxidation indicators (K232, K270, peroxide value) and higher concentration of antioxidants (total phenols and pigments). In sum, the use of bioenrichment methods could be a sustainable solution for the promotion of the quality characteristics of EVOO in Algeria.
Keywords: Bioenrichment, Satureja montana L., Essential oil, Extra virgin olive oil, Display, Quality stability.
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