This work investigates a Sentinel-2 based crop identification methodology for the monitoring of the Common Agricultural Policy's (CAP) Cross Compliance (CC) and Greening obligations. In this regard, we implemented and evaluated a parcel-based supervised classification scheme to produce accurate crop type mapping in a smallholder agricultural zone in Navarra, Spain. The scheme makes use of supervised classifiers Support Vector Machines (SVMs) and Random Forest (RF) to discriminate among the various crop types, based on a large variable space of Sentinel-2 imagery and Vegetation Index (VI) time-series. The classifiers are separately applied at three different levels of crop nomenclature hierarchy, comparing their performance with respect to accuracy and execution time. SVM provides optimal performance and proves significantly superior to RF for the lowest level of the nomenclature, resulting in 0.87 Cohen's kappa coefficient. Experiments were carried out to assess the importance of input variables, where top contributors are the Near Infrared (NIR), vegetation red-edge, and ShortWave Infrared (SWIR) multispectral bands, and the Normalized Difference Vegetation (NDVI) and Plant Senescence Reflectance (PSRI) indices, sensed during advanced crop phenology stages. The scheme is finally applied to a Lansat-8 OLI based equivalent variable space, offering 0.70 Cohen's kappa coefficient for the SVM classification, highlighting the superior performance of Sentinel-2 for this type of application. This is credited to Sentinel-2's spatial, spectral, and temporal characteristics.
The broad aim of this work was to study intraspecific variation of seed germination in Phillyrea angustifolia L. (Oleaceae), a species with a hard (water-permeable) endocarp. Germination of seeds from six different wild populations was correlated with traits related either to seed morphology or to environmental parameters. Germination of naked seeds (seeds without endocarp) at the optimum germination conditions was similar among populations and individuals, but great differences could be detected regarding the germination of seeds with endocarp both at inter- and intra-populational levels. Differences among populations could be related to climatic parameters and to morphometric variables of seeds with endocarp. A higher germination was associated with populations growing in habitats with more severe summer (higher temperature, lower precipitation and a longer drought period) and producing elongated seeds (lower Feret ratio and roundness). Moreover, seeds from eight different individuals within a population were tested independently, and great differences regarding the germination of seeds with endocarp could be detected among individuals. Our results suggest that the morphological variation found in P. angustifolia endocarp is both under strong maternal genetic control as well as influenced by environmental factors, as indicated by the high variability among individuals within one population and the significant correlation between climate variables and seed germination among populations. Finally, it is emphasized that standardization of plant propagation protocols should take into account the degree of intraspecific variation of Mediterranean species.
Aim of study: The purpose was to determine the type of dormancy and the optimal germination conditions of Phillyrea angustifolia (Oleaceae) seeds.Area of study: Germination requirements of P. angustifolia seeds collected from wild plants growing in the province of Ávila (Central Spain) were studied.Materials and methods: Seed water uptake was measured. Seeds with and without an endocarp were germinated at different temperatures, and several treatments were tested.Main results: The lignified endocarp interferes mechanically with the emergence of the radicle, and the treatments that achieved the highest germination percentages were the total removal of the endocarp with pliers (84%) or the immersion in liquid nitrogen for 1 min (97%). Scarification with concentrated sulphuric acid did not significantly increase germination compared to the control seeds, and treatments with dry heat or wet heat were detrimental to seed germination. The optimum temperature for germination was 15 ºC. A pre-sowing treatment of soaking in distilled water for 24 h slightly increased germination speed. Neither cold stratification at 5 ºC nor soaking in a gibberellic acid solution improved seed germination.Research highlights: Phillyrea angustifolia seeds have physiological dormancy -that is, the embryo does not have enough growth potential to overcome the mechanical restriction of the lignified endocarp. The seeds do not exhibit physical dormancy, given their water-permeable lignified endocarp. Our results suggest that the optimum germination protocol for P. angustifolia would be the total removal of the endocarp or immersion in liquid nitrogen for 1 min, followed by immersion in distilled water for 24 h and then seed incubation at 15 ºC in light or darkness.
SSR analyses identified an intermediate but interesting genetic diversity in 23 traditional apple cultivars grown in mountainous rural areas from central Spain. Six of them have not been cited in previous research, indicating the existence of singular and valuable germplasm in the area.
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