Similar CIN2+ sensitivity for HPV testing in first-void urine, physician-taken smear and brush-based self-sample.

Metallic Au nanowires were electrochemically synthesized in 20 µm thick ion track etched polycarbonate membranes with the nominal pore diameter of 200 nm. Scanning and transmission electron microscopy analysis and x-ray diffraction of samples revealed that the nanowires are dense with a fcc [Formula: see text] texturing. The I-V characteristics of a single Au nanowire were investigated using a four-point microprobe set-up. The Au nanowire was placed in electrical contact with electrodes patterned on planar substrates using a dual-beam focused ion beam technique. The resistivity of the Au nanowires was found to be 2.8 × 10(-4) Ω cm.

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Objective To evaluate the feasibility and utility of intraoperative post-conisation human papillomavirus (IOP-HPV) testing and cytology to detect treatment failure in patients with cervical intraepithelial neoplasia grades 2-3 (CIN2-3).Design Prospective observational pilot study.Setting Barcelona, Spain.Population A cohort of 132 women treated for CIN2-3 by loop electrosurgical conisation.Methods An endocervical sample was obtained intraoperatively with a cytobrush from the cervix remaining after the conisation. The material was kept in PreservCyt medium and processed for Hybrid Capture 2 and cytology. Patients were followed-up for 24 months. The performance of IOP-HPV testing and IOP cytology was compared with conventional indicators of recurrence (cone margin, endocervical curettage, and HPV testing and cytology at 6 months).Main outcome measure Treatment failure (i.e. recurrent CIN2-3 during follow-up).Results Treatment failure was identified in 12 women (9.1%). IOP-HPV testing for sensitivity, specificity, and positive and negative predictive values for treatment failure were 91.7, 78.3, 62.2, and 96.0%, respectively, which are similar to the figures for conventional HPV testing at 6 months (91.7, 76.0, 64.0, and 95.1%, respectively), and are better than the values of other conventional predictive factors (cone margin, endocervical curettage, and cytology intraoperative at 6 months). IOP-HPV was strongly associated with treatment failure in the multivariate analysis (OR 15.40, 95% CI 1.58-150.42).Conclusion IOP-HPV testing is feasible, and accurately predicts treatment failure in patients with CIN2-3. This new approach may allow an early identification of patients with treatment failure, thereby facilitating the scheduling of an attenuated follow-up for negative patients who are at very low risk of persistent disease.Keywords Cervical intraepithelial neoplasia, conisation, human papillomavirus, post-treatment follow-up.Please cite this paper as: Torné A, Fusté P, Rodríguez-Carunchio L, Alonso I , del Pino M, Nonell R, Cardona M, Rodríguez A, Castillo P, Pahisa J, Balasch J, Ramírez J, Ordi J. Intraoperative post-conisation human papillomavirus (IOP-HPV) testing for early detection of treatment failure in patients with cervical intraepithelial neoplasia: a pilot study. BJOG 2013;120:392-399.