The inhalation of pollutants of automobile origin induces blood disturbances, especially noncommunicable diseases, in humans. However, there are no studies highlighting the effects induced by car pollutants. From this perspective, this study aims to evaluate the effects of at least two (02) pollutants, carbon monoxide (CO) and nitrogen dioxide (NO 2), on oxidative stress and inflammation after a single exposure for four hours (04 h). Materials and methods: Ten male Wistar strain rats were randomly divided into two groups of five (05): the control group (179.00 ± 5.916 g) unexposed and the group exposed to CO and NO 2 (188.00 ± 13.13 g). CO and NO 2 were produced by the combustion gas oil using a device contained in a sealed metal box supplied with ambient air by a pump. The ranges of CO and NO 2 concentrations to which the rats were exposed varied between 35 and 45 ppm and between 0.2 and 0.3 ppm, respectively. The blood samples were taken at 24 hours after the end of the manipulations. A malondialdehyde (MDA) assay was performed on supernatant using the GENESYS 10S UV Visible Spectrophotometer. Tumour Necrosis factor alpha (TNF-α) levels in the supernatant stored at-80 ° C were examined with an ELISA kit. Results: The mean values of MDA (17,771 ± 6.624 nM / ml) and TNF-α (83,050 ± 7.483 μg / ml) observed in the exposed group were significantly higher (p ˂ 0.01) than those recorded in the control group (MDA (7.097 ± 1.882 nM / ml) and TNF-α (6.410 ± 3.160 μg / ml). Conclusion: Exposure to CO and NO 2 for at least 4 hours induces the overproduction of reactive oxygen species (ROS) and the exacerbation of inflammation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.