El Virus del Papiloma Humano (HPV), posee una predilección por los tejidos poliestratificados, con persistencia en capas basales, de allí que a partir de ello es el agente etiológico del cáncer de cérvix, principalmente. Este tipo de cáncer es el segundo más frecuente en mujeres, alrededor del mundo. El HPV es único agente infeccioso oncogénico que lo provoca. Se realizó un trabajo para determinar la prevalencia del virus en mujeres, utilizando técnicas de detección de ADN mediante PCR, en tiempo real, a partir de biopsia de cérvix, además de establecer las características histopatológicas y clínicas relacionadas. La prevalencia fue del 30,67%, la presentación histopatológica más frecuentemente infectada fue el Cáncer de cérvix y el in situ. Mientras más displásica es la histopatología del cérvix, más frecuente es la presentación del virus. Se demostró una asociación entre la exposición a la infección y la presencia de secreción vaginal blanquecina y la dispareunia. ABSTRACT Human Papilloma Virus (HPV) has a predilection for poly-stratified tissues, with persistence in the basal layers, hence mainly is the etiological agent of cervical cancer. Cervical cancer is the second most common cancer in women worldwide. HPV is unique oncogenic infectious agent that causes it. The objective of the study was to determine the prevalence of the virus in women, using DNA detection techniques through PCR, in real-time, from the cervical biopsy. In addition, the related histopathological and clinical characteristics were established. The prevalence was 30.67%, the most frequently infected histopathology presentation was cervical and in situ cancer. The more dysplastic the histopathology of the cervix, the more frequent the presentation of the virus. An association between exposure to infection and the presence of whitish vaginal discharge and dyspareunia was demonstrated.
This study aimed to elucidate, for the first time, the effects of chronical oral treatment with zinc chloride on the depressant‐like behavior of Wistar rats and correlate them with the neuroendocrine profile promoted by our protocol. Two groups of male Wistar rats (200‐250 g) were treated with zinc chloride (15 mg/kg n=16) or isotonic saline (n=17) during 30 days. The rats were kept in a controlled room temperature with light/dark cycle and food ad libitum. After the treatment, both groups were submitted to a behavioral assessment in an open field (OF), elevated plus maze (EPM) and forced swim (FS) test for five minutes. Moreover, basal plasma corticosterone, triiodothyronine (T3), thyroxine (T4) were measured. Also, iodothyronine deiodinase type 2 (Dio2) activity in brown adipose tissue (BAT) and hippocampus were assessed. In view of our results, chronic treatment with orally zinc chloride does not alter the spontaneously locomotor activity as assessed by OF. However, zinc‐treated group showed anxiolytic effects as shown by less time of grooming (P<0,05), even though the episodes of grooming did not differ between groups. Corroborating the aforementioned anxiolytic effects on OF, zinc treatment reduced the time spent in closed arms (P<0,01) and the number of fecal pellets (P<0,05) on EPM. Yet, zinc‐treated group showed fewer immobilization time compared to control group on FS (P<0,05). Regarding to neuroendocrine profile, zinc‐treated group showed higher plasmatic T3 (P<0,01), however there were no differences on serum corticosterone and T4. Furthermore, there were no differences on Dio2 activity on BAT and hippocampus. We can conclude that chronically treatment with orally zinc chloride provides antidepressant‐like effects and discrete anxiolytic‐like effects during adulthood, presumably by raising plasma T3 level with no involvement of Dio2, taking into account that rodents with signs of depression‐like behavior often present hypothyroidism.
Grant Funding Source: FAPERJ/CNPq
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