RESUMO -Montrichardia linifera, conhecida popularmente como aninga, pertence à família Araceae e forma grandes populações às margens dos rios e igarapés da Amazônia, principalmente de águas brancas (barrentas). Seus frutos fazem parte da dieta alimentar de peixes, tartarugas e de grandes herbívoros, como peixe-boi e búfalo, havendo, portanto, a hipótese de seu aproveitamento na nutrição animal. Entretanto, apesar de sua vasta distribuição na paisagem amazônica, não existem dados na literatura sobre a composição nutricional desses frutos. Com os objetivos de avaliar o seu potencial nutricional e contribuir para o conhecimento dessa espécie, foi realizada, neste trabalho, a caracterização física, química e nutricional dos frutos. A infrutescência pesa em média 500 g, com um conjunto de aproximadamente 80 frutos. O seu valor nutritivo se resume, basicamente, ao seu valor energético (≅ 350 kcal), devido principalmente ao teor de carboidratos (≅ 80%), possuindo baixo valor proteico (< 0,5%). As concentrações de manganês (≅ 1.800 mg kg -1 ) foram consideradas tóxicas, extrapolando o limite máximo tolerável em nutrição de bubalinos (1.000 mg kg -1 ).Palavras-chave: Amazônia, macrófita aquática, manganês, Montrichardia linifera, nutrição animal.ABSTRACT -Montrichardia linifera, commonly known as aninga, belongs to the family Araceae and forms large populations on the banks of rivers and streams of the Amazon, especially in white water (muddy). Its fruit are part of the diet of fish, turtles and large herbivores, such as manatees and buffalo, thus they can be used in animal nutrition. However, despite its wide distribution in the Amazon landscape, there are no published data on the nutritional composition of this fruit. To assess its nutritional potential and to contribute to the knowledge of this species, this work aimed to carry out its physical, chemical, and nutritional characterization. Infructescence on average weighs 500 g with a set of about 80 fruits. Its nutritional value consists basically in its energy value (≅ 350 kcal), due mainly to the carbohydrate content (≅ 80%), presenting low protein value (< 0.5%). Manganese concentrations (≅ 1.800 mg kg -1 ) were considered toxic, far exceeding the maximum tolerable in nutrition of buffaloes (1.000 mg kg -1 ).
RESUMOÉ apresentada uma relação de Poaceae coletadas em áreas inundáveis e inundadas do nordeste do Estado do Pará. São registrados 31 gêneros, compreendendo 83 espécies. Os grupos mais bem representados são os gêneros Paspalum e Panicum (17 e 18 taxa, respectivamente) e a tribo Paniceae (57 taxa). São fornecidos posição sistemática, comentários sobre afinidades taxonômicas, distribuição geográfica, ciclo de vida, coleções botânicas e ilustração da espigueta das espécies.
The use bulbs of Eleutherine bulbosa for treatament of diseases caused by malaria, amoeba and bacteria. This study accomplished the botanical and phytochemical characterization, antimicrobial activity and cytotoxic of E. bulbosa. For the anatomical studies, the studied material was fixed in formaldehyde – acetic acid and ethanol and it was dyed in safranin and in astra blue. For histochemistry, was fixed in buffered neutral formalin and in ferrous sulphate in formalina. Ethanolic Extract (EE) was submitted to fractionation in a chromatographic column and four (4) fractions were obtained from it. The Dichloromethane Fraction (DF) was submitted to a new fractionation. The biological activity was evalueted by diffusion in agar, microdilution and celular viability MTT. The bulb of E. bulbosa is characterized by a reduced caulinar axis and by succulent amiliferous cataphylls, epidermis with the presence of anthocyanins, homogeneous mesophyll with idioblasts of prismatic crystals of calcium oxalate and phenolic compounds. In agar diffusion it was observed that EE, DF and ethyl acetate fraction (ACF) were active for Staphylococcus aureus. In microdilution, DF (Inhibitory minimum concentration= 125 µg/ mL) was more active. For all samples the Minimous Bactericidal Concentration was >1000 µg/ mL. The fractionation contributes positively with the citotoxicity, being subfractions S1 and S2 the most citotoxic ones. The Fraction Dichloromethane was the most active one for S. aureus and more citotoxicity to VERO cells. Probably the less citotoxicity of EE is related to the presence of anthocyanins that are present on bulbs epidermis.
Grown-love (Portulaca pilosa L) is used in Amazon medicine for the treatment of diseases caused by bacteria, wound care. The objective of this work was to conduct a botanical survey of P. pilosa, as well as to evaluate its leishmanicidal activity. For the botanical studies techniques of epidermal decoupling and histological sections were used (by hand and microtome). The ethanol extract (EE) of aerial parts of P. pilosa was obtained by maceration, followed by concentration in evaporator route. The EE was fractionated by chromatographic column. The EE and fractions were analyzed in thin layer chromatography (TLC) and NMR. The leishmanicidal activity was evaluated against promastigotes and amastigotes of Leishmania amazonensis, and promastigotes of L. chagasi, with prior cytotoxicity assessment in murine macrophages. In anatomical terms, P. pilosa presents leaf surface with adaxial epidermal cells and sinuous walls. In the phytochemical prospection of the extract, terpenes and phenols were detected, among others. The analysis of the NMR suggested the presence of terpenes. All samples were inactive against promastigotes and amastigotes of L. (L) amazonensis. The dichloromethane fraction was active against promastigotes of L. (L) chagasi. The EE, dichloromethane, ethyl acetate and methanol fractions showed no cytotoxicity to murine peritoneal macrophages (CC50> 500 mg/mL). The dichloromethane fraction was promising for Leishmania, so futher studies may lead to the isolation of the active metabolite.
Lettuce is a consumed crop in the world, and increased yield is a desirable trait, and can be realized with the use of biopromotors, already described in many cultures. The objective of this work was to test the ability of two rhizobacterial isolates P. fluorescens (BRM-3211) and B. pyrrocinia (BRM-3213) to promote anatomical and growth changes in lettuce plants in the seedling stage. The increments promoted by B. pyrrocinia were smaller when using P. fluorescens. The BRM-32111 isolate, promoted increases in leaf area of 133%, 31% and 307% of fresh shoot and root mass, also increased 51% and 143% of aerial and root dry mass. The plants inoculated with BRM-32111 increased in 100% and 68% of the aerial part and radicular compared to the control treatment, and induced alterations in the anatomical characteristics in the root in 74% in the radicular diameter, 20% of the thickness of the cortex, 67% of the exoderme and 171% of the endoderm. In the leaf anatomy, BRM 32111 increased by 18% in the parenchyma expansion, 56% in the number of parenchyma cell layers, 122% adaxial endoderm, 23% epidermis, 174% beam expansion, 37% when compared to control. In this way the use of BRM-32111 influences the growth and morphology of lettuce seedlings.
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