BackgroundThe agriculturally important pasture grass tall fescue (Festuca arundinacea Schreb. syn. Lolium arundinaceum (Schreb.) Darbysh.) is an outbreeding allohexaploid, that may be more accurately described as a species complex consisting of three major (Continental, Mediterranean and rhizomatous) morphotypes. Observation of hybrid infertility in some crossing combinations between morphotypes suggests the possibility of independent origins from different diploid progenitors. This study aims to clarify the evolutionary relationships between each tall fescue morphotype through phylogenetic analysis using two low-copy nuclear genes (encoding plastid acetyl-CoA carboxylase [Acc1] and centroradialis [CEN]), the nuclear ribosomal DNA internal transcribed spacer (rDNA ITS) and the chloroplast DNA (cpDNA) genome-located matK gene. Other taxa within the closely related Lolium-Festuca species complex were also included in the study, to increase understanding of evolutionary processes in a taxonomic group characterised by multiple inter-specific hybridisation events.ResultsPutative homoeologous sequences from both nuclear genes were obtained from each polyploid species and compared to counterparts from 15 diploid taxa. Phylogenetic reconstruction confirmed F. pratensis and F. arundinacea var. glaucescens as probable progenitors to Continental tall fescue, and these species are also likely to be ancestral to the rhizomatous morphotype. However, these two morphotypes are sufficiently distinct to be located in separate clades based on the ITS-derived data set. All four of the generated data sets suggest independent evolution of the Mediterranean and Continental morphotypes, with minimal affinity between cognate sequence haplotypes. No obvious candidate progenitor species for Mediterranean tall fescues were identified, and only two putative sub-genome-specific haplotypes were identified for this morphotype.ConclusionsThis study describes the first phylogenetic analysis of the Festuca genus to include representatives of each tall fescue morphotype, and to use low copy nuclear gene-derived sequences to identify putative progenitors of the polyploid species. The demonstration of distinct tall fescue lineages has implications for both taxonomy and molecular breeding strategies, and may facilitate the generation of morphotype and/or sub-genome-specific molecular markers.

Plantago lanceolata L. is a herb species with a broad distribution in grasslands throughout the temperate world. The leaf is highly palatable to grazing animals, providing a mineral- rich forage. The species is rapid to establish, grows on a wide range of agricultural soils and is tolerant of drought and of many common diseases and pests. Two productive upright cultivars of plantain have been bred, Grasslands Lancelot and the more erect winter active Ceres Tonic. Plantain contains a range of biologically active compounds, often in large quantities. The antimicrobial compounds present can inhibit rumen fermentation and change the volatile fatty acid composition of the rumen. These changes have potential to affect bloat, animal performance and milk composition. The performance of animals grazing plantain has varied from excellent in mixed pastures to poor on nitrogen-fertilised pure swards. Animals grazing plantain have been observed to have a reduced incidence of scouring and dags in some trials, but despite a mild anthelmintic effect detected in the laboratory, field trials have failed to detect any significant reduction in worm burdens. The most likely use of plantain on farms is as a component of mixed pasture swards. Its contribution is likely to be greatest where grass growth is less vigorous and where there are gaps in the sward. These conditions are likely to be found in low fertility dryland pastures. It is unlikely to be the dominant species and could be expected to contribute less than 20% of the sward, except where the grass or legume growth is poor. Keywords: perennial forage herbs, Plantago lanceolata, plantain

No abstract

Perennial ryegrass (Lolium perenne) cultivars adapted to New Zealand conditions are bred by several scientifically equipped New Zealand interests. New cultivars are evaluated in a network of trials organised by the New Zealand Plant Breeding and Research Association. This paper presents data from 17 trials completed throughout New Zealand since 1991. Data were analysed for each trial and then in a multi-site analysis enabling comparison of estimated cultivar means, over all New Zealand trials, North Island trials and Canterbury trials. New cultivars released to the market yielded on average 6% more herbage annually, and 9% in summer, than cultivars available before 1991. Other cultivars were evaluated but showed no yield advantage and have not been commercially released. Point analysis of plots in the final season of trials indicated that recently released cultivars maintain ground cover at least as well as the older ones. Limited data for rust infection indicated that the oldest cultivars are the most severely affected. Keywords: cultivar evaluation, endophyte, NZPBRA, pasture plant breeding, perennial ryegrass

Lolium perenne L. (perennial ryegrass) is the most important pasture grass species in temperate regions of the world. However, its growth is restricted in summer dry environments. Germplasm screening can be used to identify accessions or individual plants for incorporation into breeding programs for drought tolerance. We selected nine perennial ryegrass accessions from different global origins and from a range of climatic and environmental conditions. In addition, the perennial ryegrass cultivar ‘Grasslands Impact’ was chosen as a reference. The accessions were grown for 360 days in a controlled environment through six consecutive drought stress and recovery cycles. We observed intraspecific differences in drought stress responsiveness for shoot biomass and survival from the third stress cycle. An accession from Norway had 50% more shoot dry matter than the next best-performing accession after six drought cycles. Compared with the reference cultivar ‘Grasslands Impact’, shoot dry matter of the accession from Norway was more than seven times higher after six drought cycles, indicating superior performance of this ecotype under drought stress. Drought tolerance was characterized by osmotic adjustment and higher relative leaf water content at low soil moisture levels. Furthermore, the findings of this study identify solute potential as an early predictor of drought stress tolerance. These intraspecific differences can be used in breeding programs for the development of drought-tolerant perennial ryegrass cultivars.