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ObjectiveTo define preliminary guidelines for the use of lymphatic mapping techniques in patients with breast cancer. Summary Background DataLymphatic mapping techniques have the potential of changing the standard of surgical care of patients with breast cancer. MethodsFour hundred sixty-six consecutive patients with newly diagnosed breast cancer underwent a prospective trial of intraoperative lymphatic mapping using a combination of vital blue dye and filtered technetium-labeled sulfur colloid. A sentinel lymph node (SLN) was defined as a blue node and/or a hot node with a 10:1 ex vivo gamma probe ratio of SLN to non-SLN. All SLNs were bivalved, step-sectioned, and examined with routine hematoxylin and eosin (H&E)

Once individuals are diagnosed with melanoma, they are in a high-risk population for having other primary site melanomas diagnosed and should be placed in an intensive follow-up protocol consisting of a complete skin examination.

SummaryA piezoelectric quartz crystal microbalance has been modified for the deterniination of antibody activity in solution. Antigen precoated crystals are exposed to antisera of varying concentration and specificity. Only antisera specific for the antigen precoat will form an additional protein layer on the crystal. The thickness of the antibody layer, measured by the frequency shift of the crystal, is proportional to the concentration of specific antibody in solution. The terhnique provides a rapid qualitative and quantitative assay of antibody activity.