Blood parameters are an important index to assess the body homeostasis in mammals. Here, the effect of pesticide (imidacloprid) contaminated feed exposure on the haematological parameters of adult rabbits (n=15) was studied. Pesticides (Imidacloprid, Bildor® 0.5ml (100mg)/L water spray on vegetables and green grass) exposed feed fed to rabbits with wash (washed feed rabbit) or without a wash (not washed feed rabbit) in the once-daily morning for every alternative day up to 15 days. Control rabbits have received a standard diet (fresh vegetables and green grass). The blood cell analysis showed that the total erythrocyte count, packed cell volume, and haemoglobin values were not changed significantly in pesticide exposed rabbit. For differential leukocyte count, the percentage of neutrophil and eosinophil was significantly decreased in the pesticide-exposed rabbit as compared to the control rabbit. The lymphocyte percentage was increased significantly in pesticide-exposed rabbits compared to control rabbits. Interestingly, the number of lymphocytes was significantly increased in not washed feed rabbits compared to wash feed rabbits. The results of the present study suggest that residue of agriculturally used pesticides may affect the body homeostasis of animals.
Res. Agric., Livest. Fish.7(3): 439-444, December 2020
The liver consists of parenchymal hepatocytes and non‐parenchymal cells. Non‐parenchymal cells, Kupffer cells, hepatic stellate cells and cholangiocytes have crucial roles in liver homeostasis and liver pathology. To establish baseline data, this study investigated immunohistochemically the distribution of non‐parenchymal cells in perivenular areas (PV), periportal areas (PP) and Glisson's sheath (GS) of adult rat liver. Liver tissues were collected from the left lateral lobe of rats. CD163‐positive macrophages were seen along the sinusoid of PV and PP areas, indicating Kupffer cells. Double immunofluorescence showed, Kupffer cells partly co‐expressed CD68 and MHC class II antigens in the liver. The numbers of Kupffer cells were significantly high in PP areas as compared with PV or GS areas. CD68‐positive exudative macrophages were highly localized in PP and GS areas and a comparatively low PV area. MHC class II‐positive dendritic cells (activated macrophages) were localized mainly in GS. Granzyme B‐positive NK cells were mainly localized in the Glisson's sheath. CD3‐positive T cells and CD20‐positive B cells were distributed along the sinusoids of the PP and PV areas of hepatic lobules. Vimentin and glial fibrillary acidic protein (GFAP)‐positive hepatic stellate cells were localized along sinusoids in the hepatic lobules of the liver. Cholangiocytes reacting to cytokeratin 19 were seen on interlobular bile ducts in Glisson's sheath of the liver. This study shows that heterogeneous macrophage populations, liver‐resident lymphocytes and hepatic stellate cells localized in PP and PV areas or GS areas of the liver with cells specific patterns.
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