The use of superior and adequate sugarcane seeds are being an important factor either in tissue culture orquickly provision of seeds (Single Bud Planting). This study aims to determine the influence of Hot Water Treatment (HWT) temperature and auxin on the initial growth of sugarcane. The experiment was arranged factorially by using Completely Randomized Design which was repeated three times. The first factor of HWT immersion temperature comprising: without immersion and immersion with temperature of 30 ° C, 45 ° C, and 50 ° C; the second factor of auxin consisting of: without auxin, atonic, and onion extract. The variables that observed such as appearance and length of bud eye, plant height, number of leaves, stem diameter, root length and weight, wet weight of fiberplant. The data were analysed by ANOVA which was tested with 5% HSD test. The results showed that HWT temperature immersion and auxin administration, respectively, influenced the length of bud eye, plant height, number of leaves, stem diameter, wet weight of plant, weight and root length up to 60 days after treatment. There is an interaction effect between auxin administration and immersion of HWT towards shoot bud length, plant height, leaf number, wet weight; and immersion with HWT temperature of 50°C for 10 minutes and atonic treatment giving the best result. Thus, the treatment can be applied to sugarcane nursery.