Introduction:Monocytes play a major role in eliciting the immune response against infection. Also mast cells are found to play a critical role in mediating inflammatory immune response not only to allergic reaction but also to infection by inducing the production of inflammatory cytokines such as IL-1β, IL-6, IL-18 and IL-17. This study aims to investigate nod like receptor protein 3 (NLRP3) mediated production of Th1/Th17 cytokines in monocytes and mast cells. Materials and Methods:Mononuclear cells from peripheral and cord blood were stimulated with lipopolysaccharide (LPS), peptidoglycan (PGN) whereas human mast cell line-1 (HMC-1) cells were stimulated with histamine and LPS to analyse the activation of NLRP3 inflammosome components such as NLRP3, procaspase-1(p45), caspase-1(p10) nuclear factor-κB (NF-κB) and extracellular signal regulated kinase (ERK). The release of cytokines such as IL-1β, IL-6, IL-18 and IL-17 was quantified.Results: This study shows that stimulating the mononuclear and mast cells with LPS, PGN and LPS, histamine respectively induce the production of IL-1β, IL-6, IL-18 and IL-17 whereas the costimulation with gram positive and gram negative bacterial stimulants such as LPS and PGN showed synergistic response to cytokines whereas HMC-1 cells when stimulated with histamine produces IL-1β, IL-6 and IL-17 but when co-stimulated with LPS level of cytokines were reduced. It is mediated through activation of NLRP3 inflammosome. Also these cells utilized NFκB and ERK pathway. Conclusion:The activation of NLRP3 inflammosome in innate immune cells leads to the production of IL-1β, IL-6, IL-18 and IL-17 cytokine. The signaling pathways NF-κB and ERK are found to have been involved in the activation of NLRP3 inflammosome complex during inflammatory conditions. ÖzGiriş: Monositler, infeksiyona karşı gelişen bağışıklık yanıtında çok önemli bir rol oynar. Aynı zamanda, mast hücreleri de sadece allerjik reaksiyonlara değil aynı zamanda IL-1β, IL-6, IL-17 ve IL-18 gibi sitokinlerin üretilmesi ile infeksiyon bağışıklığında da kritik öneme sahiptir. Bu çalışmada düğüm benzeri reseptör proteini 3 (İng; 'nod like receptor protein 3;NLRP3)'e bağlı olarak monosit ve mast hücrelerinden salınan Th1/Th12 sitokinleri araştırılmıştır. Gereç ve Yöntemler:Periferik kandan ve kordon kanından elde edilen mononükleer hücreler, lipopolisakkarid (LPS), peptidoglikan (PGN) ile uyarılır iken insan mast hücresi hattı-1 (HMC-1) hücreleri histamin ve LPS ile uyarılmış ve bu uyarılmanın NLRP3, prokaspaz-1(p45), kaspaz-1(p10), nükleer faktör κB(NF-κB) ve ERK gibi inflamazon bileşenlerinin salınımına olan etkisi irdelendi. IL-1β, IL-6, IL-17 ve IL-18 gibi sitokinlerin salınımı ölçüldü.Bulgular: Bu çalışma, mononükleer hücreler ve mast hücrelerinin LPS, PGN, LPS ve uyarılması ile IL-1β, IL-6, IL-17 ve IL-18 uyarılır iken, bu hücrelerin LPS ve PGN gibi gram-negatif ve gram-pozitif bakterilerin uyarıya neden olan molekülleri ile birlikte uyarıldıklarından daha yüksek bir sinerjik uyarım gerçekleşti, HMC-1 hücreleri histamin ile uyar...
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