The objective of this study was the development, optimization, and validation of a RP-HPLC method for the quantification of reduced glutathione (GSH) and oxidized glutathione (GSSG) in pharmaceutical formulations The separation utilized a C18 column at room temperature with absorption wavelength 210nm. The mobile phase was an isocratic flow of a 95:5 (v/v) mixture of 25mM phosphate buffer (pH 2.7) and methanol with flow rate at 1.0 mL/min. Validation of the method assessed with the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. The method show an acceptable degree of linearity with r²=0.9994 and 0.999 over a concentration range of 10-200 μg/mL for GSH and GSSG respectively. The detection limit and quantification limit for GSH 20.7μg/mL and 69.24μg/mL and for GSSG 17.22μg/mL and 57.42μg/mL respectively. The percent recovery of the method was 99.98-100.93 %. Following validation, the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a liposome. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.
Fourier transform infrared spectroscopy is an effectual and noncritical approach for quantitative study of different types analytes present in pharmaceutical and foodstuffs. The present review describes the basic principles and the instrumentation of FTIR spectroscopy along with its sample preparation techniques, sample handling techniques and advancements. FTIR spectroscopy in combination with chemometrics techniques has been followed over long times. The main objective of this review is to assemble the data linked to application of FTIR spectroscopic and chemometrics techniques for the quantitative study of varieties of analytes like API, adulterants, caffeine, cocaine, lipids, fats & oils, sugar and others. The FTIR spectroscopy with chemometrics techniques proved to be a beneficial methodology for quantitative study to routine analysis of these analytes.
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